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December 2012 Volume 6 Issue 6

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Article

Effect of recombinant adeno-associated BMP‑4/7 fusion gene on the biology of BMSCs

  • Authors:
    • Shao-Hui Yuan
    • Zhenggang Bi
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, the First Hospital of Harbin Medical University, Harbin 150001, P.R. China
  • Pages: 1413-1417
    |
    Published online on: September 20, 2012
       https://doi.org/10.3892/mmr.2012.1090
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Abstract

The aim of the study was to investigate the effect of recombinant adeno-associated virus (AAV) with morphogenetic protein 4/7 fusion gene on the ossification of rabbit bone marrow stromal cells (BMSCs). The genes BMP-4 and BMP-7 were obtained through one-step reverse transcriptase polymerase chain reaction from human placental cells. The BMP-4/7 fusion gene was generated through recombination. The rabbit BMSCs were transfected with the recombinant AAV vectors carrying AAV-BMP-4/7 with various multiplicity of infection (MOI) values. Expression of fusion gene BMP-4/7 protein was determined by the ELISA method. The ossification of cells was evaluated by observing alkaline phosphatase (ALP) and osteocalcin (OC) activity after transfection for 7 and 14 days. We successfully constructed the recombinant AAV with the BMP-4/7 fusion gene. A 29-20 kDa protein was shown by SDS-PAGE electrophoresis following transfection with AAV-BMP-4/7. The fusion protein BMP-4/7 in BMSCs transferred by AAV showed a positive correlation with various MOI values. There was significantly higher ALP and OC activity in the AAV-BMP-4/7 transfection groups than in the AAV-EGFP groups (tALP=896.88, P<0.001, tOC=543.24, P<0.01). The fusion gene BMP-4/7 is capable of enhancing the expression of BMPs and possesses significant ossification activity through AAV.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan S and Bi Z: Effect of recombinant adeno-associated BMP‑4/7 fusion gene on the biology of BMSCs. Mol Med Rep 6: 1413-1417, 2012.
APA
Yuan, S., & Bi, Z. (2012). Effect of recombinant adeno-associated BMP‑4/7 fusion gene on the biology of BMSCs. Molecular Medicine Reports, 6, 1413-1417. https://doi.org/10.3892/mmr.2012.1090
MLA
Yuan, S., Bi, Z."Effect of recombinant adeno-associated BMP‑4/7 fusion gene on the biology of BMSCs". Molecular Medicine Reports 6.6 (2012): 1413-1417.
Chicago
Yuan, S., Bi, Z."Effect of recombinant adeno-associated BMP‑4/7 fusion gene on the biology of BMSCs". Molecular Medicine Reports 6, no. 6 (2012): 1413-1417. https://doi.org/10.3892/mmr.2012.1090
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan S and Bi Z: Effect of recombinant adeno-associated BMP‑4/7 fusion gene on the biology of BMSCs. Mol Med Rep 6: 1413-1417, 2012.
APA
Yuan, S., & Bi, Z. (2012). Effect of recombinant adeno-associated BMP‑4/7 fusion gene on the biology of BMSCs. Molecular Medicine Reports, 6, 1413-1417. https://doi.org/10.3892/mmr.2012.1090
MLA
Yuan, S., Bi, Z."Effect of recombinant adeno-associated BMP‑4/7 fusion gene on the biology of BMSCs". Molecular Medicine Reports 6.6 (2012): 1413-1417.
Chicago
Yuan, S., Bi, Z."Effect of recombinant adeno-associated BMP‑4/7 fusion gene on the biology of BMSCs". Molecular Medicine Reports 6, no. 6 (2012): 1413-1417. https://doi.org/10.3892/mmr.2012.1090
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