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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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December 2012 Volume 6 Issue 6

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Article

Decreased levels of nitric oxide production and nitric oxide synthase-2 expression are associated with the development and metastasis of hepatocellular carcinoma

  • Authors:
    • Lei Zhou
    • Ying Wang
    • De-An Tian
    • Juan Yang
    • Yu-Zhen Yang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R. China
  • Pages: 1261-1266
    |
    Published online on: September 24, 2012
       https://doi.org/10.3892/mmr.2012.1096
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Abstract

Many studies have demonstrated the function of nitric oxide (NO) or nitric oxide synthase-2 (NOS-2) in cancer as pro-neoplastic or anti-neoplastic effectors, but the role of NO and NOS-2 in hepatocellular carcinoma (HCC) remains unclear. The aim of this study was to investigate the levels of NO production and NOS-2 expression in HCC and adjacent non-tumor liver tissues and to clarify whether the levels of NO/NOS-2 are related to the clinicopathological features of HCC. The levels of NO production were examined in tumor and adjacent non-tumor liver tissues of 30 patients with HCC. The expression of NOS-2 was detected by real-time polymerase chain reaction (RT-PCR) and immunohistochemical analysis in HCC and/or adjacent non-tumor liver tissues. Mutant p53 and proliferating cell nuclear antigen (PCNA) were also immunohistochemically investigated in liver tissues. The levels of NO in HCC were significantly lower compared to adjacent non-tumor liver tissues (P<0.001). The relative mRNA and protein expression levels of NOS-2 in HCC were also significantly lower compared to adjacent non-tumor liver tissues (P<0.01 for both). We found that the levels of NO in patients suffering from HCC metastasis were lower compared to those without metastasis (P<0.05) and NOS-2 expression was correlated with tumor diameter (P<0.05) and metastasis (P<0.05). In addition, mutant p53 protein was expressed in the majority of HCC samples and the proliferation rate of HCC was significantly higher than that of adjacent non-tumor liver tissues. These data indicate that decreased levels of NO/NOS-2 may partially contribute to overexpression of the mutant p53 protein and excessive proliferation; this may be a potential mechanism in the development and progression of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou L, Wang Y, Tian D, Yang J and Yang Y: Decreased levels of nitric oxide production and nitric oxide synthase-2 expression are associated with the development and metastasis of hepatocellular carcinoma. Mol Med Rep 6: 1261-1266, 2012.
APA
Zhou, L., Wang, Y., Tian, D., Yang, J., & Yang, Y. (2012). Decreased levels of nitric oxide production and nitric oxide synthase-2 expression are associated with the development and metastasis of hepatocellular carcinoma. Molecular Medicine Reports, 6, 1261-1266. https://doi.org/10.3892/mmr.2012.1096
MLA
Zhou, L., Wang, Y., Tian, D., Yang, J., Yang, Y."Decreased levels of nitric oxide production and nitric oxide synthase-2 expression are associated with the development and metastasis of hepatocellular carcinoma". Molecular Medicine Reports 6.6 (2012): 1261-1266.
Chicago
Zhou, L., Wang, Y., Tian, D., Yang, J., Yang, Y."Decreased levels of nitric oxide production and nitric oxide synthase-2 expression are associated with the development and metastasis of hepatocellular carcinoma". Molecular Medicine Reports 6, no. 6 (2012): 1261-1266. https://doi.org/10.3892/mmr.2012.1096
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou L, Wang Y, Tian D, Yang J and Yang Y: Decreased levels of nitric oxide production and nitric oxide synthase-2 expression are associated with the development and metastasis of hepatocellular carcinoma. Mol Med Rep 6: 1261-1266, 2012.
APA
Zhou, L., Wang, Y., Tian, D., Yang, J., & Yang, Y. (2012). Decreased levels of nitric oxide production and nitric oxide synthase-2 expression are associated with the development and metastasis of hepatocellular carcinoma. Molecular Medicine Reports, 6, 1261-1266. https://doi.org/10.3892/mmr.2012.1096
MLA
Zhou, L., Wang, Y., Tian, D., Yang, J., Yang, Y."Decreased levels of nitric oxide production and nitric oxide synthase-2 expression are associated with the development and metastasis of hepatocellular carcinoma". Molecular Medicine Reports 6.6 (2012): 1261-1266.
Chicago
Zhou, L., Wang, Y., Tian, D., Yang, J., Yang, Y."Decreased levels of nitric oxide production and nitric oxide synthase-2 expression are associated with the development and metastasis of hepatocellular carcinoma". Molecular Medicine Reports 6, no. 6 (2012): 1261-1266. https://doi.org/10.3892/mmr.2012.1096
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