Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells

Li,Hong-Liang; Zhang,Shu; Wang,Yuan; Liang,Rong-Rui; Li,Jun; An,Peng; Wang,Zi-Ming; Yang,Jun; Li,Zong-Fang

doi:10.3892/mmr.2012.1123

Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells

Authors:
- Hong-Liang Li
- Shu Zhang
- Yuan Wang
- Rong-Rui Liang
- Jun Li
- Peng An
- Zi-Ming Wang
- Jun Yang
- Zong-Fang Li
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Affiliations: Department of Urology, Second Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, P.R. China, Engineering Research Center of Biotherapy and Translational Medicine of Shaanxi Province, Xi'an Jiaotong University, Xi'an 710004, P.R. China, Department of Pathology, Shaanxi Provincial People's Hospital, Xi'an 710068, P.R. China
Published online on: October 9, 2012 https://doi.org/10.3892/mmr.2012.1123
Pages: 266-270

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Abstract

Recurrence of bladder cancer following transurethral resection of bladder tumor (TURBt) is an obstacle in clinical management. In the current study, we investigated the antitumor activity of baicalein, a Chinese herbal medicine, against T24 bladder cancer cells in vitro. Baicalein inhibited growth and caused G1/S arrest of the cell cycle in the T24 cells. Moreover, baicalein induced apoptosis via loss of mitochondrial transmembrane potential (ΔΨm), release of cytochrome c and activation of caspase-9 and caspase-3. Baicalein inhibited Akt phosphorylation, downregulated Bcl-2 expression and upregulated Bax expression, which in turn increased the ratio of Bax/Bcl-2. Our results demonstrate that baicalein repressed growth inhibition and induced apoptosis via loss of ΔΨm and activation of caspase-9 and caspase-3 in T24 bladder cancer cells, which indicates that baicalein may be an effective agent in the clinical management of bladder cancer.

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