Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells

  • Authors:
    • Liang-Liang Sun
    • Le Zhang
    • Xiang-Lan Meng
    • Feng Zhang
    • Yun Zhao
    • Xin Jin
  • View Affiliations

  • Published online on: October 22, 2012     https://doi.org/10.3892/mmr.2012.1137
  • Pages: 110-114
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Abstract

To investigate the molecular mechanisms of laminar shear stress on the inhibition of apoptosis in endothelial cells, human umbilical vein endothelial cells (HUVECs) were starved in medium containing 2% fetal bovine serum (FBS) and treated with 15 dyne/cm2 shear stress. We confirmed that 15 dyne/cm2 shear stress inhibited the expression of Omi/HtrA2 at the mRNA and protein levels in cultured HUVECs. Furthermore, the release of Omi/HtrA2 from the mitochondria was induced by removal of basic fibroblast growth factor and decrease of FBS in the medium, while shear stress inhibited its release under the same conditions. These results suggest that downregulation of Omi/HtrA2 may contribute to the potent anti-atherosclerotic effect of shear stress by preventing endothelial cells from entering apoptosis.
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January 2013
Volume 7 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Sun L, Zhang L, Meng X, Zhang F, Zhao Y and Jin X: Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells. Mol Med Rep 7: 110-114, 2013
APA
Sun, L., Zhang, L., Meng, X., Zhang, F., Zhao, Y., & Jin, X. (2013). Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells. Molecular Medicine Reports, 7, 110-114. https://doi.org/10.3892/mmr.2012.1137
MLA
Sun, L., Zhang, L., Meng, X., Zhang, F., Zhao, Y., Jin, X."Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells". Molecular Medicine Reports 7.1 (2013): 110-114.
Chicago
Sun, L., Zhang, L., Meng, X., Zhang, F., Zhao, Y., Jin, X."Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells". Molecular Medicine Reports 7, no. 1 (2013): 110-114. https://doi.org/10.3892/mmr.2012.1137