mRNA expression of interleukins and Th1/Th2 imbalance in patients with pulmonary embolism
- Authors:
- Qianglin Duan
- Wei Lv
- Lemin Wang
- Zhu Gong
- Qiang Wang
- Haoming Song
- Hao Wang
View Affiliations
Affiliations: Department of Cardiology, Tongji Hospital of Tongji University, Shanghai 200065, P.R. China
- Published online on: October 23, 2012 https://doi.org/10.3892/mmr.2012.1142
-
Pages:
332-336
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Abstract
Few studies have investigated the changes of Th1- and Th2-type cytokines in pulmonary embolism (PE) patients. In this study, the gene expression of interleukins and the balance of Th1- and Th2-type cytokines in the peripheral blood mononuclear cells (PBMCs) of PE patients and controls were investigated. A total of 20 PE patients and 20 gender- and age-matched controls were included in the study. Human cDNA microarray analysis was used to detect the differences in cytokine gene expression between the two groups and a random variance model corrected t-test was used to analyze the statistical data. In comparison with the controls, 12 genes were found to be downregulated, specifically IL1A, IL9, IL17B, IL19, IL23A, IL25 (p<0.05), IL2, IL3, IL13, IL22, IL24 and IL31 (p<0.01), and 2 genes were found to be upregulated, specifically IL10 and IL28A, in the PE patients. The expression levels of IFN-γ and IL2 mRNA in the PE patients were significantly lower than those in the control group (p<0.01), while the IL20 mRNA expression levels were significantly upregulated (p<0.01). We conclude that there are significant differences in interleukin gene expression between the PE patients and the control group. A shift of the Th1/Th2 balance comprising enhanced Th2 activity and reduced Th1 activity in the PE patients is also demonstrated.
View References
|
1
|
Imberti D, Ageno W, Manfredini R, et al:
Interventional treatment of venous thromboembolism: a review.
Thromb Res. 129:418–425. 2012. View Article : Google Scholar : PubMed/NCBI
|
|
2
|
Heil JA: The epidemiology of venous
thromboembolism in the community. Arterioscler Thromb Vasc Biol.
28:370–372. 2008. View Article : Google Scholar : PubMed/NCBI
|
|
3
|
Haoming S, Lemin W, Zhu G, et al: T
cell-mediated immune deficiency or compromise in patients with
CTEPH. Am J Respir Crit Care Med. 183:417–418. 2011. View Article : Google Scholar : PubMed/NCBI
|
|
4
|
Xiang-Hua Y, Le-Min W, Ai-Bin L, et al:
Severe acute respiratory syndrome and venous thromboembolism in
multiple organs. Am J Respir Crit Care Med. 182:436–437. 2010.
View Article : Google Scholar : PubMed/NCBI
|
|
5
|
Xiao W, Yu H and Zheng C: The imbalance of
Th1/Th2 cytokine expression in peripheral blood mononuclear cell
from asthmatic patients and the effect of erythromycin on these
cytokines. Zhonghua Jie He He Hu Xi Za Zhi. 23:347–350. 2000.(In
Chinese).
|
|
6
|
Constant SL and Bottomly K: Induction of
Th1 and Th2 CD4+ T cell responses: the alternative
approaches. Annu Rev Immunol. 15:297–322. 1997. View Article : Google Scholar : PubMed/NCBI
|
|
7
|
Downing LJ, Strieter RM, Kadell AM, et al:
IL-10 regulates thrombus-induced vein wall inflammation and
thrombosis. J Immunol. 161:1471–1476. 1998.PubMed/NCBI
|
|
8
|
Fox EA and Kahn SR: The relationship
between inflammation and venous thrombosis. A systematic review of
clinical studies. Thromb Haemost. 94:362–365. 2005.PubMed/NCBI
|
|
9
|
Wang L, Gong Z, Liang A, et al:
Compromised T-cell immunity and virus-like structure in a patient
with pulmonary hypertension. Am J Respir Crit Care Med.
182:434–435. 2010. View Article : Google Scholar : PubMed/NCBI
|
|
10
|
Gong Z, Liang AB, Wang LM, et al: The
expression and significance of immunity associated genes mRNA in
patients with pulmonary embolism. Zhonghua Nei Ke Za Zhi.
48:666–669. 2009.(In Chinese).
|
|
11
|
Wang L, Song H, Gong Z, Duan Q and Liang
A: Acute pulmonary embolism and dysfunction of CD3+
CD8+ T cell immunity. Am J Respir Crit Care Med.
184:13152011. View Article : Google Scholar : PubMed/NCBI
|
|
12
|
Schmidt M, Horvath-Puho E, Thomsen RW,
Smeeth L and Sørensen HT: Acute infections and venous
thromboembolism. J Intern Med. 271:608–618. 2012. View Article : Google Scholar : PubMed/NCBI
|
