Expression and the clinical significance of hPTTG1 in gastric cancer
- Authors:
- Published online on: November 1, 2012 https://doi.org/10.3892/mmr.2012.1161
- Pages: 43-46
Abstract
Introduction
Pituitary tumor-transforming gene (PTTG) was first identified in rat pituitary tumor by differential display PCR (1). At present, three members of the PTTG family have been identified in humans (2). Among those, the hPTTG1 has a human securin function, while playing an important role in tumorigenesis and tumor metastasis (3,4). The hPTTG1 was found to be overexpressed in a variety of cancers, such as lung cancer, thyroid tumor, as well as ovarian and esophageal cancer (5–8). Normally, the hPTTG1 expression can only be detected in testis, thymus, embryo and liver (4). The abnormal expressions of hPTTG1 implied tumorigenesis or tumor metastasis. However, the correlation between the expression of hPTTG1 and gastric cancer has yet to be clarified.
Cancer has been the leading cause of mortality in many countries (9,10), with gastric cancer being the second leading cause of cancer-related mortality in both males and females worldwide (11). Gastric cancer is still widespread in China, as seen in a statistical report demonstrating that China accounts for approximately 50% of the global gastric cancer burden (11). It is widely accepted that early discovery indicates a good prognosis in cancer therapy. Thus, it is urgent to develop a facilitating technique to detect tumors, at least tumor metastasis.
The hPTTG1 gene has transcriptional activity and securin functions (12). Overexpression of the hPTTG1 promotes lymph node metastasis in human esophageal carcinoma (8). Findings of previous studies revealed that the downregulation of the expression of hPTTG1 resulted in the suppression of tumor growth (13,14). We hypothesized that the abnormal overexpression of hPTTG1 was involved in tumorigenesis and tumor metastases in gastric cancer.
Lymph node metastasis is an important prognostic factor in early gastric cancer, which indicates the risk of recurrence (15). Thus it is necessary to detect whether lymph node metastasis is present prior to curative resection. The removal of lymph nodes should be carried out during surgery. Therefore, a reliable method is needed to accurately detect lymph node metastasis in primary gastric cancer. However, the expression and correlation of hPTTG1 with clinicopathological parameters in gastric cancer has not been reported yet. Therefore, SP immunohistochemical technology was applied to investigate the expression of hPTTG1 in gastric cancer and adjacent normal tissues and explore its correlation with clinicopathological features and significance.
The aim of the present study was to develop a facilitation method to detect gastric cancer or lymph node metastasis in gastric cancer. We found that the expression of hPTTG1 was associated with gastric cancer and lymph node metastasis in gastric cancer. We suggested that the immunohistochemistry staining of hPTTG1 may be used as an effective tool to reveal its clinicopathological parameters and significance in gastric cancer.
Materials and methods
Patients and tissues
Gastric cancer and adjacent normal tissues were collected at the time of surgery from patients with gastric cancer between May, 2004 and May, 2008. The tumor samples were confirmed by histopathological examination. Prior to surgery, the patients did not receive radiation treatment, chemo-treatment or biological therapy for the tumor. The adjacent normal tissues were obtained from a site 5 cm away from the tumor and confirmed histologically. These samples were obtained from 28 males and 17 females. The average age was 59.5 years (range, 45–79). The tumor-node-metastasis (TNM) stages were classified according to the Union for International Cancer Control (UICC) 1987 of TNM classification. Five cases of stage I, 10 cases of stage II, 20 cases of stage III and 10 cases of stage IV were involved in our study. Of the 45 cases, 31 had a lymph node metastasis, and 14 cases were not detected at surgery. In the present study, there were 16 cases with well-differentiated, 15 with moderately-differentiated and 14 cases with poorly-differentiated adenocarcinoma.
Immunohistochemistry
An SP immunohistochemical staining kit was purchased from Beijing Zhongshan Bio-tech Co., Ltd. Immunohistochemistry was performed according to the manufacturer's instructions. Briefly, gastric cancer tissues and adjacent normal tissues were dissected out, followed by fixing in Bouin's solution for 24 h. After an extensive wash in 70% ethanol, the samples were dehydrated and embedded in paraffin. The paraffin blocks were cut at 5 μm. The deparaffinized sections were incubated with a 3% hydrogen peroxide solution for 30 min. After being washed with PBS solution twice, the slides with sample sections were incubated with 0.01 M citrate solution at 95°C for 10 min to retrieve the epitope. After cooling to room temperature, the glass slides were rinsed with PBS solution twice. The slides were incubated with NGS-PBS (PBS containing 10% normal goat serum) for 30 min, and then washed with PBS solution.
Primary antibody, rabbit anti-human PTTG1 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) at a dilution of 1:100 was dropped onto the slides. After incubation at 37°C for 1 h the slides were washed with PBS solution twice. Subsequently, the secondary antibody (biotin-conjugated goat anti-rabbit IgG, Beijing Zhongshan Bio-tech Co., Ltd., China) at a dilution of 1:100 was dropped onto the slides and incubated at 37°C for 30 min. After washing with PBS solution twice, the slides were applied to 3,3′-diaminobenzidine (Dako Tech Co., Ltd,, Denmark) staining. The positive slides were defined according to a previous study (16). Briefly, the slides were defined as i) (+) if from 10–25% of highest possible frequency (HPF), showing a positive signal with a magnification of ×400; ii) (++) if from 25–50% of HPF, showing positive signal with magnification at ×400 and iii) (+++) if >50% of HPF, showing positive signal with a magnification of ×400. Each slide was examined by 2 pathologists, respectively. In case of a disagreement, the slide was determined by a third pathologist.
Statistical analyses
Data were presented as the mean ± SD. Statistical analysis was performed using SPSS 11.0.0 (SPSS Inc., Chicago). Correlations were evaluated using the Chi-square test. p<0.05 was considered to indicate a statistically significant difference.
Results
Expression of hPTTG1 in gastric cancer and the adjacent normal tissues
We detected the expression of hPTTG1 in different cell types and tissues (Fig. 1). A number of nuclei exhibited dark staining in signet-ring cell carcinoma, poorly differentiated adenocarcinoma and adjacent normal tissues, possibly indicating a poor prognosis. The statistical analysis revealed that the expression of hPTTG1 was associated with gastric cancer as compared to the adjacent normal tissues (Table I).
Correlation between hPTTG1 expression and the clinicopathological variables of gastric cancer
The statistical analysis demonstrated that the expression of hPTTG1 was associated with differentiation levels, clinical classification and lymph node metastasis in gastric cancer. This finding suggests that the overexpression of hPTTG1 may indicate poor prognosis. Moreover, we found that the expression of hPTTG1 did not correlate with gender, age or pathological types (Table II).
Discussion
The abnormal expression of hPTTG1 is involved in tumorigenesis and tumor metastasis (17). Our study showed that the immunohistochemical analysis of hPTTG1 was suitable for detecting different cell types and tissues in gastric cancer. We also showed that the expression of hPTTG1 is correlated with gastric cancer as compared to the adjacent normal tissues. This finding indicated that the expression of hPTTG1 may be a useful reference to gastric cancer detection.
Results from our study demonstrated that the expression of hPTTG1 was associated with differentiation levels, clinical classification and lymph node metastasis in primary gastric cancer. Poorly differentiated cancer and higher clinical classification indicated poor prognosis. Thus, this finding suggests that the expression of hPTTG1 is associated with poor prognosis. Lymph node metastasis is the most important prognostic factor for patients with early gastric cancer. Since the presence of lymph node metastasis indicates a poor prognosis on patients with early gastric cancer, it is suggested that the first and second tier lymph nodes be removed during surgery (15). The removal of lymph nodes was reported to be associated with an extended period of survival (18). Therefore, the detection of lymph node metastasis is valuable for early gastric cancer patients. We suggest that the expression of hPTTG1 be considered in early gastric cancer therapy.
A statistical report of 2008 showed that age-standardized incidence rates are approximately twice as high in men as in women (11). The expression of hPTTG1, however, did not correlate with gender. Moreover, we found that the expression of hPTTG1 was also not associated with age or pathological types.
Our results describe hPTTG1 expression as a potential tool for the assessment of tumor aggressiveness. The immunohistochemistry of hPTTG1 expression may be a convenient way to large-scale analyses or routine pathological diagnosis. This tool may have special value for early gastric cancer patients, for the detection of lymph node metastasis and extention of their period of survival.
Acknowledgements
This study was supported by a grant from the National Special Funds for the Health Research Foundation of China (no. 200802112) and the National Natural Science Foundation of China (no. 81071991).
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