Association of pre-miRNA-146a rs2910164 and pre‑miRNA-499 rs3746444 polymorphisms and susceptibility to rheumatoid arthritis

Hashemi,Mohammad; Eskandari-Nasab,Ebrahim; Zakeri,Zahra; Atabaki,Mahdi; Bahari,Gholamreza; Jahantigh,Mahdi; Taheri,Mohsen; Ghavami,Saeid

doi:10.3892/mmr.2012.1176

Association of pre-miRNA-146a rs2910164 and pre‑miRNA-499 rs3746444 polymorphisms and susceptibility to rheumatoid arthritis

Authors:
- Mohammad Hashemi
- Ebrahim Eskandari-Nasab
- Zahra Zakeri
- Mahdi Atabaki
- Gholamreza Bahari
- Mahdi Jahantigh
- Mohsen Taheri
- Saeid Ghavami
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Affiliations: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran, Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran, Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran, Department of Immunology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran, Department of Pathology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran, Genetics of Non Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran, Department of Physiology, Manitoba Institute of Child Health, University of Manitoba, Winnipeg, MB, Canada
Published online on: November 8, 2012 https://doi.org/10.3892/mmr.2012.1176
Pages: 287-291

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Abstract

Single nucleotide polymorphisms in pre‑microRNA (miRNA) may alter miRNA expression levels or processing and contribute to susceptibility in a wide range of diseases. The present study aimed to evaluate the possible association between rs2910164 and rs3746444 of the pre-miRNA (hsa-mir-146a and hsa-mir-499) polymorphisms and susceptibility to rheumatoid arthritis (RA) in an Iranian population. This case-control study was performed on 104 patients with RA and 110 healthy individuals. Tetra amplification refractory mutation system-polymerase chain reaction was used to genotype the hsa-mir-499 rs3746444 and hsa-mir-146a rs2910164 polymorphisms. The hsa-mir-499 rs3746444 polymorphism was a risk factor for predisposition to RA in codominant [TT vs. TC: odds ratio (OR), 2.11; 95% confidence interval (CI), 1.08-4.11; p=0.029; TT vs. CC: OR, 3.88; 95% CI, 1.68-8.98; p=0.002], dominant (TT vs. TC-CC: OR, 2.64; 95% CI, 1.48-4.72; p=0.001) and recessive (TC-CC vs. CC: OR, 3.05; 95% CI, 1.36-6.83; p=0.007) tested inheritance models. In addition, the rs3746444 C allele was a risk factor for RA (OR, 2.49; 95% CI, 1.63-3.81; p<0.0001). No significant difference was found between the groups concerning the rs2910164 polymorphism (χ2=0.348, p=0.841). Our findings demonstrated that the hsa-mir-499 rs3746444, but not mir-146a rs2910164, polymorphism is associated with an increased RA risk in a sample of the Iranian population. Larger studies with different ethnicities are required to validate our findings.

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