Expression of pancreatic regenerating gene in lung and intestinal tissue correlates with the severity of disease in rats with acute necrotizing pancreatitis

Hu,Hao-Lin; Zhang,Qi; Kong,Bo; Shi,Xin

doi:10.3892/mmr.2012.1187

Expression of pancreatic regenerating gene in lung and intestinal tissue correlates with the severity of disease in rats with acute necrotizing pancreatitis

Authors:
- Hao-Lin Hu
- Qi Zhang
- Bo Kong
- Xin Shi
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Affiliations: Department of General Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China
Published online on: November 16, 2012 https://doi.org/10.3892/mmr.2012.1187
Pages: 503-508

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Abstract

The aim of this study was to investigate the expression pattern of regenerating gene I (Reg I) in the lung and intestinal tissues of rats with acute necrotizing pancreatitis (ANP), as well as the correlation of Reg I expression with lung and intestinal injury. Sprague-Dawley rats were randomly allocated to control (n=40) and ANP (n=80) groups. The rats in the control group received laparotomy only. In the ANP group, 3% sodium taurocholate was injected into the pancreatic duct to develop the ANP model. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect the Reg I mRNA levels in the pancreas, intestine and lung. The pathological changes in the pancreas, intestine and lung were observed and the serum amylase levels, the wet/dry weight ratio of the lung and the permeability of the intestinal mucosa were measured. The measured parameters were found to correlate with the Reg I mRNA levels. Reg I mRNA was more highly expressed in the pancreas, intestine and lung in the ANP rats than in the control group. The Reg I expression levels were positively correlated with the pathological scores, serum amylase levels, lung pathological scores, lung tissue wet/dry ratios, intestinal pathological scores and intestinal permeability. The levels of Reg I were increased in the lung and intestinal tissue of the ANP rats and the expression levels of Reg I correlated closely with the severity of the lung and intestinal injury.

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1	Hartwig W, Werner J, Müller CA, Uhl W and Büchler MW: Surgical management of severe pancreatitis including sterile necrosis. J Hepatobiliary Pancreat Surg. 9:429–435. 2002. View Article : Google Scholar : PubMed/NCBI
2	Appelros S and Borgström A: Incidence, aetiology and mortality rate of acute pancreatitis over 10 years in a defined urban population in Sweden. Br J Surg. 86:465–470. 1999.PubMed/NCBI
3	Ammori BJ, Leeder PC, King RF, Barclay GR, Martin IG, Larvin M and McMahon MJ: Early increase in intestinal permeability in patients with severe acute pancreatitis: correlation with endotoxemia, organ failure, and mortality. J Gastrointest Surg. 3:252–262. 1999. View Article : Google Scholar : PubMed/NCBI
4	Okamoto H: The Reg gene family and Reg proteins: with special attention to the regeneration of pancreatic beta-cells. J Hepatobiliary Pancreat Surg. 6:254–262. 1999. View Article : Google Scholar : PubMed/NCBI
5	Zhang YW, Ding LS and Lai MD: Reg gene family and human diseases. World J Gastroenterol. 9:2635–2641. 2003.PubMed/NCBI
6	Zheng HC, Sugawara A, Okamoto H, Takasawa S, Takahashi H, Masuda S and Takano Y: Expression profile of the REG gene family in colorectal carcinoma. J Histochem Cytochem. 59:106–115. 2011. View Article : Google Scholar : PubMed/NCBI
7	Planas R, Pujol-Autonell I, Ruiz E, Montraveta M, Cabre E, Lucas-Martin A, Pujol-Borrell R, Martinez-Caceres E and Vives-Pi M: Regenerating gene Iα is a biomarker for diagnosis and monitoring of celiac disease: a preliminary study. Transl Res. 158:140–145. 2011.
8	Bluth MH, Patel SA, Dieckgraefe BK, Okamoto H and Zenilman ME: Pancreatic regenerating protein (reg I) and reg I receptor mRNA are upregulated in rat pancreas after induction of acute pancreatitis. World J Gastroenterol. 12:4511–4516. 2006.PubMed/NCBI
9	Lin YY, Viterbo D, Mueller CM, Stanek AE, Smith-Norowitz T, Drew H, Wadgaonkar R, Zenilman ME and Bluth MH: Small-interference RNA gene knockdown of pancreatitis-associated proteins in rat acute pancreatitis. Pancreas. 36:402–410. 2008. View Article : Google Scholar : PubMed/NCBI
10	Viterbo D, Callender GE, DiMaio T, Mueller CM, Smith-Norowitz T, Zenilman ME and Bluth MH: Administration of anti-Reg I and anti-PAPII antibodies worsens pancreatitis. JOP. 10:15–23. 2009.PubMed/NCBI
11	Yekebas EF, Strate T, Zolmajd S, et al: Impact of different modalities of continuous venovenous hemofiltration on sepsis-induced alterations in experimental pancreatitis. Kidney Int. 62:1806–1818. 2002. View Article : Google Scholar
12	Park PO, Haglund U, Bulkley GB and Fält K: The sequence of development of intestinal tissue injury after strangulation ischemia and reperfusion. Surgery. 107:574–580. 1990.PubMed/NCBI
13	Sun SL, Wu SD and Zhang XB: Oral (99m)Tc-DTPA simultaneous determination of duodenobiliary reflux and intestinal permeability in patients after choledocholithotomy plus T-tube drainage. Hepatobiliary Pancreat Dis Int. 4:593–596. 2005.PubMed/NCBI
14	Schmidt J, Rattner DW, Lewandrowski K, Compton CC, Mandavilli U, Knoefel WT and Warshaw AL: A better model of acute pancreatitis for evaluating therapy. Ann Surg. 215:44–56. 1992. View Article : Google Scholar : PubMed/NCBI
15	Bimmler D, Schiesser M, Perren A, Scheele G, Angst E, Meili S, Ammann R and Graf R: Coordinate regulation of PSP/reg and PAP isoforms as a family of secretory stress proteins in an animal model of chronic pancreatitis. J Surg Res. 118:122–135. 2004. View Article : Google Scholar : PubMed/NCBI
16	Boeck L, Graf R, Eggimann P, et al: Pancreatic stone protein: a marker of organ failure and outcome in ventilator-associated pneumonia. Chest. 140:925–932. 2011. View Article : Google Scholar : PubMed/NCBI
17	Zenilman ME, Zheng QH, Wu H and Rengabashyam P: Pancreatic Reg and a conserved bioactive fragment are mitogenic through the MAPK P38 pathway. Surg Forum. 191(Suppl 1): S292000.PubMed/NCBI
18	Sekikawa A, Fukui H, Fujii S, et al: REG Ialpha protein may function as a trophic and/or anti-apoptotic factor in the development of gastric cancer. Gastroenterology. 128:642–653. 2005. View Article : Google Scholar : PubMed/NCBI
19	Imaoka H, Ishihara S, Kazumori H, et al: Exacerbation of indomethacin-induced small intestinal injuries in Reg I-knockout mice. Am J Physiol Gastrointest Liver Physiol. 299:G311–G319. 2010. View Article : Google Scholar : PubMed/NCBI
20	Kawanami C, Fukui H, Kinoshita Y, Nakata H, Asahara M, Matsushima Y, Kishi K and Chiba T: Regenerating gene expression in normal gastric mucosa and indomethacin-induced mucosal lesions of the rat. J Gastroenterol. 32:12–18. 1997. View Article : Google Scholar : PubMed/NCBI
21	Dieckgraefe BK, Crimmins DL, Landt V, Houchen C, Anant S, Porche-Sorbet R and Ladenson JH: Expression of the regenerating gene family in inflammatory bowel disease mucosa: Reg Ialpha upregulation, processing, and antiapoptotic activity. J Investig Med. 50:421–434. 2002. View Article : Google Scholar : PubMed/NCBI