Silencing P12CDK2AP1 with a lentivirus promotes HaCaT cell proliferation

Sun,Moyi; Zheng,Jun; Xue,Hui; Jiang,Yuegui; Li,Chunnan; Li,Jianhu; Jin,Wei; Shen,Minzhi; Yang,Xiangming; Ni,Qianwei

doi:10.3892/mmr.2012.1205

Silencing P12CDK2AP1 with a lentivirus promotes HaCaT cell proliferation

Authors:
- Moyi Sun
- Jun Zheng
- Hui Xue
- Yuegui Jiang
- Chunnan Li
- Jianhu Li
- Wei Jin
- Minzhi Shen
- Xiangming Yang
- Qianwei Ni
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Affiliations: Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Conservative Dentistry, School of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Biochemistry and Molecular Biology and The State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
Published online on: November 27, 2012 https://doi.org/10.3892/mmr.2012.1205
Pages: 471-475

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Abstract

The tumor suppressor P12CDK2AP1 negatively regulates cyclin-dependent kinase 2 (CDK2) activities and suppresses DNA replication. Notably, P12CDK2AP1 is known to be downregulated in head and neck squamous cell carcinomas (HNSCCs). Silencing of specific gene expression by small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) using expression vectors and retroviruses has become a powerful tool for the genetic analysis of mammalian cells. In the present study, we utilized lentivirus‑mediated shRNA for functional gene knockdown in normal human skin keratinocytes (HaCaT) cells in order to assess the potential role of P12CDK2AP1 in HNSCCs. Lentivirus‑mediated RNA interference (RNAi) effectively reduced endogenous P12CDK2AP1 expression in HaCaT cells and significantly promoted HaCaT cell proliferation in vitro. Lentiviral vectors have the ability to infect dividing and non-dividing cells as well as to achieve long‑term multilineage gene expression. Thus, additional studies are needed to investigate the use of such vectors as a therapeutic tool for the delivery of siRNAs.

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