Interleukin-10 gene modification attenuates hepatocyte activation of rat hepatic stellate cells in vitro

  • Authors:
    • Yun-Xin Chen
    • Yue-Hong Huang
    • Wei-Da Zheng
    • Zhi-Xin Chen
    • Li-Juan Zhang
    • Xiao-Zhong Wang
  • View Affiliations

  • Published online on: December 11, 2012     https://doi.org/10.3892/mmr.2012.1228
  • Pages: 371-378
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Abstract

Activation of hepatic stellate cells (HSCs) plays a key role in the progression of liver fibrosis. Interleukin-10 (IL-10), a potential anti-fibrosis cytokine, has an unfavorable pharmacokinetic profile, which limits its clinical applications. A liver-targeting gene delivery system may maintain a longer-lasting concentration in hepatic tissue with fewer side‑effects in non-target tissues. In the present study, when delivered by asialoglycoprotein receptor-mediated liposomes, the IL-10 gene was highly expressed in BRL cells (a rat hepatocyte line) and attenuated the apoptosis of BRL cells induced by plasmid transfection. In a co-culture system, BRL cells demonstrated a marked ability to stimulate the proliferation of primary HSCs and their expression of α-SMA and procollagen type I. Following modification of the BRL cells with the IL-10 gene, this stimulation was attenuated and an accelerated apoptosis of the HSCs was induced. These results suggest that hepatocyte‑targeting gene delivery may be an ideal technique for the IL-10 gene therapy of liver fibrosis, which requires further confirmation by in vivo studies.
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February 2013
Volume 7 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Chen Y, Huang Y, Zheng W, Chen Z, Zhang L and Wang X: Interleukin-10 gene modification attenuates hepatocyte activation of rat hepatic stellate cells in vitro. Mol Med Rep 7: 371-378, 2013.
APA
Chen, Y., Huang, Y., Zheng, W., Chen, Z., Zhang, L., & Wang, X. (2013). Interleukin-10 gene modification attenuates hepatocyte activation of rat hepatic stellate cells in vitro. Molecular Medicine Reports, 7, 371-378. https://doi.org/10.3892/mmr.2012.1228
MLA
Chen, Y., Huang, Y., Zheng, W., Chen, Z., Zhang, L., Wang, X."Interleukin-10 gene modification attenuates hepatocyte activation of rat hepatic stellate cells in vitro". Molecular Medicine Reports 7.2 (2013): 371-378.
Chicago
Chen, Y., Huang, Y., Zheng, W., Chen, Z., Zhang, L., Wang, X."Interleukin-10 gene modification attenuates hepatocyte activation of rat hepatic stellate cells in vitro". Molecular Medicine Reports 7, no. 2 (2013): 371-378. https://doi.org/10.3892/mmr.2012.1228