TRESK gene recombinant adenovirus vector inhibits capsaicin-mediated substance P release from cultured rat dorsal root ganglion neurons

  • Authors:
    • Jun Zhou
    • Shang-Long Yao
    • Cheng-Xiang Yang
    • Ji-Ying Zhong
    • Han-Bing Wang
    • Yan Zhang
  • View Affiliations

  • Published online on: February 3, 2012     https://doi.org/10.3892/mmr.2012.778
  • Pages: 1049-1052
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Abstract

The present study was conducted to determine whether the activation of TRESK in the dorsal root ganglion (DRG) by the TRESK gene recombinant adenovirus vector inhibits the capsaicin-evoked substance P (SP) release using a radioimmunoassay. TRESK is an outwardly rectifying K+ current channel that contributes to the resting potential and is the most important background potassium channel in DRG. Previous studies have shown that neuropathic pain (NP) is closely related to the regulation of certain potassium channels in DRG neurons, while DRG-released SP is important in the peripheral mechanism of NP. In the present study, the TRESK gene adenovirus vector significantly enhanced the TRESK mRNA and protein of the cultured rat DRG neurons. Radioimmunoassay analysis revealed that the capsaicin‑mediated SP release was significantly inhibited by the TRESK gene recombinant adenovirus vector in rat DRG neurons. These findings suggest that TRESK plays a role in adjusting the release of SP in DRG, which is related to NP.
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April 2012
Volume 5 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhou J, Yao S, Yang C, Zhong J, Wang H and Zhang Y: TRESK gene recombinant adenovirus vector inhibits capsaicin-mediated substance P release from cultured rat dorsal root ganglion neurons. Mol Med Rep 5: 1049-1052, 2012.
APA
Zhou, J., Yao, S., Yang, C., Zhong, J., Wang, H., & Zhang, Y. (2012). TRESK gene recombinant adenovirus vector inhibits capsaicin-mediated substance P release from cultured rat dorsal root ganglion neurons. Molecular Medicine Reports, 5, 1049-1052. https://doi.org/10.3892/mmr.2012.778
MLA
Zhou, J., Yao, S., Yang, C., Zhong, J., Wang, H., Zhang, Y."TRESK gene recombinant adenovirus vector inhibits capsaicin-mediated substance P release from cultured rat dorsal root ganglion neurons". Molecular Medicine Reports 5.4 (2012): 1049-1052.
Chicago
Zhou, J., Yao, S., Yang, C., Zhong, J., Wang, H., Zhang, Y."TRESK gene recombinant adenovirus vector inhibits capsaicin-mediated substance P release from cultured rat dorsal root ganglion neurons". Molecular Medicine Reports 5, no. 4 (2012): 1049-1052. https://doi.org/10.3892/mmr.2012.778