microRNA-146a targets the L1 cell adhesion molecule and suppresses the metastatic potential of gastric cancer

Hou,Zhibo; Yin,Haitao; Chen,Cong; Dai,Xinzheng; Li,Xiaolin; Liu,Baorui; Fang,Xuefeng

doi:10.3892/mmr.2012.946

microRNA-146a targets the L1 cell adhesion molecule and suppresses the metastatic potential of gastric cancer

Authors:
- Zhibo Hou
- Haitao Yin
- Cong Chen
- Xinzheng Dai
- Xiaolin Li
- Baorui Liu
- Xuefeng Fang
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Affiliations: Department of Oncology, The Affiliated Zhongda Hospital of Southeast University, Nanjing, Jiangsu 210029, P.R. China , Department of Gynecology of Traditional Chinese Medicine, Jiangsu Provincial Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu 210029, P.R. China , Department of Liver Surgery, Liver Transplantation Center of Jiangsu Province, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China , Department of Geriatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China , The Comprehensive Cancer Center of Drum Tower Hospital, Nanjing University, Nanjing, Jiangsu 210008, P.R. China , Department of Medical Oncology, Cancer Institute, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, P.R. China
Published online on: June 13, 2012 https://doi.org/10.3892/mmr.2012.946
Pages: 501-506

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Abstract

Recent studies have shown that microRNA-146a (miR-146a) is associated with cancer metastasis. However, the mechanisms underlying this process remain poorly understood. In this study, we aimed to investigate the potential role of miR-146a in gastric cancer metastasis. A wound-healing assay and a Transwell assay were used to investigate the impact of miR-146a on the migratory and invasive abilities of MKN-45 cells in vitro. MKN-45 cells stably expressing miR-146a or the negative control were transplanted into nude mice through the lateral tail vein to explore the effect of miR-146a on tumor metastasis in vivo. A luciferase reporter assay and western blot analysis were used to identify the potential target genes. Our results show that the overexpression of miR-146a inhibits the invasion and metastasis of MKN-45 cells in vitro and in vivo. Furthermore, the L1 cell adhesion molecule (L1CAM) was identified as a novel target of miR‑146a in gastric cancer. Taken together, our results provide evidence that miR-146a suppresses gastric cancer cell invasion and metastasis in vitro and in vivo, which may be in part due to the downregulation of L1CAM. miR-146a may have the therapeutic potential to suppress gastric cancer metastasis.

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