The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate

  • Authors:
    • Ping Huang
    • Jing Wang
    • Xi Shen
    • Qin Jiao
    • Yu Cheng
    • Bing Xie
    • Yisheng Zhong
  • View Affiliations

  • Published online on: June 13, 2012     https://doi.org/10.3892/mmr.2012.947
  • Pages: 662-666
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Abstract

The aim of this study was to investigate the effects of erythropoietin (EPO) on RhoA/Rho-associated kinase (ROCK) expression in rat retinal explants cultured with glutamate. After the retinal explants were cultured in serum-free R16 nutrient medium for 24 h, the retinal explants were divided into control (R16 nutrient medium), glutamate (R16 nutrient medium containing 5 mM/l glutamate) and glutamate + EPO (R16 nutrient medium containing 5 mM/l glutamate and 6.0 U/ml EPO) groups, and culturing was continued for another 72 h. The mRNA and protein expression of total RhoA, ROCK1 and ROCK2 in the retinal explants was examined by RT-PCR and western blotting, and active RhoA in the retinal explants was detected via GST-RBD binding and immunoblotting with an antibody specific to active RhoA. The total RhoA mRNA and protein expression did not differ substantially between the control, the glutamate and the glutamate + EPO groups. The glutamate increased the active RhoA, ROCK1 and ROCK2 expression in cultured retinal explants (P<0.05), whereas the expression of active RhoA, ROCK1 and ROCK2 in the glutamate + EPO group was significantly lower than that in the simple glutamate group (P<0.05) and similar to that in the control group. In conclusion, EPO downregulates active RhoA, ROCK1 and ROCK2 expression in retinal explants cultured with glutamate.
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September 2012
Volume 6 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Huang P, Wang J, Shen X, Jiao Q, Cheng Y, Xie B and Zhong Y: The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate. Mol Med Rep 6: 662-666, 2012
APA
Huang, P., Wang, J., Shen, X., Jiao, Q., Cheng, Y., Xie, B., & Zhong, Y. (2012). The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate. Molecular Medicine Reports, 6, 662-666. https://doi.org/10.3892/mmr.2012.947
MLA
Huang, P., Wang, J., Shen, X., Jiao, Q., Cheng, Y., Xie, B., Zhong, Y."The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate". Molecular Medicine Reports 6.3 (2012): 662-666.
Chicago
Huang, P., Wang, J., Shen, X., Jiao, Q., Cheng, Y., Xie, B., Zhong, Y."The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate". Molecular Medicine Reports 6, no. 3 (2012): 662-666. https://doi.org/10.3892/mmr.2012.947