Inhibition of S100A9 expression by propofol in monocytes of rats with endotoxemia

Li,Zhen; Zhao,Guodong; Zhou,Guobin; Wang,Yan; Wang,Qing; Ji,Jinquan; Wang,Zhipeng

doi:10.3892/mmr.2012.957

Inhibition of S100A9 expression by propofol in monocytes of rats with endotoxemia

Authors:
- Zhen Li
- Guodong Zhao
- Guobin Zhou
- Yan Wang
- Qing Wang
- Jinquan Ji
- Zhipeng Wang
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Affiliations: Department of Anesthesiology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China, Science and Education Department, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China
Published online on: June 20, 2012 https://doi.org/10.3892/mmr.2012.957
Pages: 657-661

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Abstract

The inflammatory response is a non-specific autoimmune response. Monocytes are the most important effector cells in the systemic inflammatory response. In recent years, the function of the intravenous anesthetic, propofol, in the inhibition of the inflammatory response has attracted much attention. However, the specific signal transduction mechanism related to the anti-inflammatory effect of propofol remains unclear. In this study, monocyte protein expression in rats with endotoxemia was detected using proteomic techniques before and after propofol intervention. By two-dimensional electrophoresis and mass spectrometric identification, we found that S100A9 protein expression was significantly reduced after propofol treatment. In addition, we used western blot analysis to confirm the results of two-dimensional electrophoresis. The S100A9 protein, a member of the S100A calcium-binding protein family, is closely related to the occurrence and development of inflammation. The results of this study suggest that the anti-inflammatory effect of propofol may be related to the inhibition of S100A9 protein expression.

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