Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus

Zhou,Xue; Song,Fa-Huan; He,Wen; Yang,Xiao-Yu; Zhou,Zhi-Bin; Feng,Xia; Zhou,Li-Hua

doi:10.3892/mmr.2012.976

Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus

Authors:
- Xue Zhou
- Fa-Huan Song
- Wen He
- Xiao-Yu Yang
- Zhi-Bin Zhou
- Xia Feng
- Li-Hua Zhou
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Affiliations: Department of Anesthesiology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China , Department of Anatomy, Zhong Shan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China
Published online on: July 2, 2012 https://doi.org/10.3892/mmr.2012.976
Pages: 543-546

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Abstract

A growing number of studies have shown that commonly used anesthetic agents may cause neurohistopathological changes and persistent behavioral impairments in the developing brain. The effects of sevoﬂurane, a widely used substance in pediatric anesthesia, on the developing brain have not been thoroughly analyzed thus far. In this study, Sprague-Dawley rats at postnatal day (P)7 were exposed to 2.3% sevoflurane for 6 h continuously. Six hours after exposure, hematoxylin and eosin (H&E) staining was used to determine the morphological changes in the hippocampus. The expression levels of neuronal nitric oxide synthase (nNOS) and caspase-3 in the hippocampus of the pups were determined by immunofluorescence. A single 6-h sevoflurane exposure at P7 caused morphological changes in the hippocampus. Sevoflurane also significantly increased the expression of cleaved caspase-3 and reduced the expression of the nNOS protein. These results indicate that neonatal exposure to sevoflurane causes neurohistopathological changes, apoptosis and decreases nNOS protein levels in the rat hippocampus.

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