Direct regulation of caspase‑3 by the transcription factor AP‑2α is involved in aspirin‑induced apoptosis in MDA‑MB‑453 breast cancer cells

Yan,Feng; He,Qiongzhi; Hu,Xiang; Li,Wen; Wei,Ke; Li,Li; Zhong,Yingli; Ding,Xiaofeng; Xiang,Shuanglin; Zhang,Jian

doi:10.3892/mmr.2013.1257

Direct regulation of caspase‑3 by the transcription factor AP‑2α is involved in aspirin‑induced apoptosis in MDA‑MB‑453 breast cancer cells

Authors:
- Feng Yan
- Qiongzhi He
- Xiang Hu
- Wen Li
- Ke Wei
- Li Li
- Yingli Zhong
- Xiaofeng Ding
- Shuanglin Xiang
- Jian Zhang
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Affiliations: Key Laboratory of Protein Chemistry and Development Biology of State Education Ministry of China,College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, P.R. China, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China, Model Organism Division, E‑Institutes of Shanghai Universities, Shanghai Jiao Tong University, Shanghai 200240, P.R. China
Published online on: January 2, 2013 https://doi.org/10.3892/mmr.2013.1257
Pages: 909-914

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Abstract

Aspirin has been reported to trigger apoptosis in various cancer cell lines. However, the detailed mechanisms involved remain elusive. The present study aimed to investigate whether aspirin plays a role in apoptosis of MDA-MB-453 cells. The effect of aspirin on the proliferation of human MDA-MB-453 cells breast cancer cells was evaluated using MTT assay, flow cytometry and western blotting. The present study reports that aspirin induces the apoptosis of MDA‑MB‑453 breast cancer cells which was attributed to the increased expression and activation of caspase‑3. Moreover, AP‑2α, a transcription factor highly expressed in MDA‑MB‑453 cells, was identified as a negative regulator of caspase‑3 transcription and AP‑2α was attenuated following aspirin treatment. Therefore, aspirin may increase the expression of caspase‑3 by inducing the degradation of AP‑2α, which increases activated caspase‑3 expression, thereby triggering apoptosis in MDA‑MB‑453 cells. Thus, aspirin may be used in breast cancer therapy.

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