Differential expression of estradiol and estrogen receptor α in severe preeclamptic pregnancies compared with normal pregnancies

Yin,Guowu; Zhu,Xiaoming; Guo,Chun; Yang,Yang; Han,Tao; Chen,Lei; Yin,Wen; Gao,Ping; Zhang,Huizhong; Geng,Jie; Wang,Julei; Liang,Liang

doi:10.3892/mmr.2013.1262

Differential expression of estradiol and estrogen receptor α in severe preeclamptic pregnancies compared with normal pregnancies

Authors:
- Guowu Yin
- Xiaoming Zhu
- Chun Guo
- Yang Yang
- Tao Han
- Lei Chen
- Wen Yin
- Ping Gao
- Huizhong Zhang
- Jie Geng
- Julei Wang
- Liang Liang
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Affiliations: Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, P.R. China, Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, P.R. China, Department of Obstetrics and Gynecology, Armed Police Corps Hospital, Hohhot, Inner Mongolia 010040, P.R. China, Department of Obstetrics and Gynecology, Affiliated Hospital of Xi'an Medical University, Xi'an, Shanxi 710077, P.R. China, Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, P.R. China, Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi'an, Shanxi 710032, P.R. China, Department of Medical Laboratory, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, P.R. China, Department of Teaching and Medical Administration, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, P.R. China, Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, P.R. China, Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an, Shanxi 710032, P.R. China
Published online on: January 4, 2013 https://doi.org/10.3892/mmr.2013.1262
Pages: 981-985

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Abstract

The purpose of this study was to investigate the expression of estradiol and estrogen receptor α (ESRα) in severe preeclamptic (sPE) pregnancies compared with normal pregnancies. Sera and placentas were obtained from i) patients with sPE (n=25) and ii) a normal control group (n=25) who underwent elective Cesarean deliveries. Estradiol expression was assessed by enzyme-linked immunosorbent assays (ELISAs). ESRα expression was assessed by reverse transcription polymerase chain reaction (RT-PCR) analysis and western blot analysis. In preeclamptic pregnancies, estradiol was underexpressed (P<0.05), however, ESRα mRNA and protein levels were increased significantly in comparison with normal pregnancies (P<0.05). These results show that estradiol and ESRα are deregulated in preeclamptic pregnancies, which in turn suggests the involvement of these molecules in the pathogenesis of preeclampsia.

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