Probucol inhibits hydrogen peroxide to induce apoptosis of vascular smooth muscle cells

Sheng,Lin; Jiao,Bo; Shao,Lijuan; Bi,Shaojie; Cheng,Chao; Zhang,Jingbo; Jiang,Yihao

doi:10.3892/mmr.2013.1299

Probucol inhibits hydrogen peroxide to induce apoptosis of vascular smooth muscle cells

Authors:
- Lin Sheng
- Bo Jiao
- Lijuan Shao
- Shaojie Bi
- Chao Cheng
- Jingbo Zhang
- Yihao Jiang
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Affiliations: Department of Cardiology, Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Pharmacology, College of Pharmacy, Shandong University, Jinan, Shandong 250012, P.R. China, College of Environmental and Chemical Engineering, Nanchang University, Nanchang, Jiangxi 330031, P.R. China
Published online on: January 29, 2013 https://doi.org/10.3892/mmr.2013.1299
Pages: 1185-1190

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Abstract

The aim of this study was to investigate the effect of the antioxidant probucol on hydrogen peroxide (H2O2)-induced vascular smooth muscle cell (VSMC) apoptosis. H2O2 (1 mmol/l) was used as the VSMC apoptosis-inducing agent, and 100, 10 and 1 µmol/l probucol concentrations were incubated with cells for 6 h. The cell apoptosis was observed using Annexin V-FITC, TUNEL and Hoechst 33258 staining methods. H2O2 promoted VSMC apoptosis, ASK-1 protein expression increased and Trx-l protein expression decreased. Probucol reduced H2O2-induced apoptosis in a dose-dependent manner; while ASK-1 protein expression decreased, Trx-1 protein expression increased. Probuco1 is capable of antagonizing the H2O2-induced apoptosis in VSMCs, which may be related to the decrease in ASK-1 protein expression and the increase in Trx-1 protein expression.

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