Paired box 2 induces epithelial-mesenchymal transition in normal renal tubular epithelial cells of rats

Li,Li; Wu,Yubin; Yang,Yongchang

doi:10.3892/mmr.2013.1365

Paired box 2 induces epithelial-mesenchymal transition in normal renal tubular epithelial cells of rats

Authors:
- Li Li
- Yubin Wu
- Yongchang Yang
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Affiliations: Department of Pediatric Nephrology and Rheumatology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
Published online on: March 12, 2013 https://doi.org/10.3892/mmr.2013.1365
Pages: 1549-1554

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Abstract

Epithelial‑mesenchymal transition (EMT) is considered to be a crucial stage of renal fibrosis. Previous studies have indicated that paired box 2 (PAX2) affects renal fibrosis, likely by regulating EMT. Therefore, we examined whether PAX2 directly induces normal renal tubular EMT in vitro. Recombinant PAX2 plasmid was transfected into a renal tubular epithelial cell line (NRK52E) derived from normal rats. The optimal time‑point was evaluated through fluorescence detection, western blotting and real-time polymerase chain reaction (PCR). Morphological alterations were examined using phase‑contrast microscopy. At the optimal time‑point of transfection, the expression of E-cadherin, α-smooth muscle actin (α-SMA), fibronectin and snail was analyzed by western blotting and real-time PCR. PAX2 expression in NRK52E cells transfected with PAX2 plasmid reached its peak at 72 h post‑transfection. The transfected cells became elongated. PAX2 transfection induced a fibrotic phenotype of NRK52E cells with increased expression of α-SMA, fibronectin and snail as well as decreased expression of E-cadherin. The optimal time-point for transfection efficiency was 72 h. In conclusion, PAX2 induces renal tubular mesenchymal transition in vitro.

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