In vivo dynamic monitoring of the biological behavior of labeled C6 glioma by MRI

Liao,Jichun; Xia,Rui; Liu,Ting; Feng,Hua; Ai,Hua; Song,Bin; Gao,Fabao

doi:10.3892/mmr.2013.1369

In vivo dynamic monitoring of the biological behavior of labeled C6 glioma by MRI

Authors:
- Jichun Liao
- Rui Xia
- Ting Liu
- Hua Feng
- Hua Ai
- Bin Song
- Fabao Gao
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Affiliations: Department of Radiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China, National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610065, P.R. China
Published online on: March 13, 2013 https://doi.org/10.3892/mmr.2013.1369
Pages: 1397-1402

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Abstract

Gliomas are the most common type of intracranial tumor and have the highest rate of mortality. The aims of this study were to investigate the long-term course and biological behavior of orthotopically implanted C6 gliomas and to dynamically monitor the distribution of superparamagnetic iron oxide (SPIO) nanocomposite-labeled C6 glioma cells in rats using 7.0T MRI. We observed that in the MRI of the rats implanted with SPIO-labeled cells, there were pronounced hypointense signal bands, which faded over time, but remained visible up to day 27 after implantation. We observed that the first tumors were detected as early as 2 days after implantation, presenting as slightly hyperintense regions with indefinite boundaries in the T1-weighted images (T1WIs). On the 9th day, thick tumor feeder vessels, ~0.2 mm in diameter, were observed and these increased rapidly over time. Edema was observed in the labeled and unlabeled groups in the T2WIs. Both the central hypointense signal area and the peripheral cogwheel-shaped hypointense signal band in the tumor were observed on the post-contrast T1WIs, in accordance with the necrosis observed in the photomicrographs following hematoxylin and eosin (HE) staining. In conclusion, labeling tumor cells with SPIO and performing an MRI scan dynamically monitors the development and biological behavior of glioma at a very early stage.

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1	Wrensch M, Minn Y, Chew T, Bondy M and Berger MS: Epidemiology of primary brain tumors: current concepts and review of the literature. Neuro Oncol. 4:278–299. 2002.PubMed/NCBI
2	Matsukado Y, Maccarty CS and Kernohan JW: The growth of glioblastoma multiforme (astrocytomas, grades 3 and 4) in neurosurgical practice. J Neurosurg. 18:636–644. 1961. View Article : Google Scholar : PubMed/NCBI
3	Demuth T and Berens ME: Molecular mechanisms of glioma cell migration and invasion. J Neurooncol. 70:217–228. 2004. View Article : Google Scholar : PubMed/NCBI
4	Weissleder R: Scaling down imaging: molecular mapping of cancer in mice. Nat Rev Cancer. 2:11–18. 2002. View Article : Google Scholar : PubMed/NCBI
5	Zhang F, Xie J, Liu G, He Y, Lu G and Chen X: In vivo MRI tracking of cell invasion and migration in a rat glioma model. Mol Imaging Biol. 13:695–701. 2011. View Article : Google Scholar : PubMed/NCBI
6	Shapiro EM, Skrtic S, Sharer K, Hill JM, Dunbar CE and Koretsky AP: MRI detection of single particles for cellular imaging. Proc Natl Acad Sci USA. 101:10901–10906. 2004. View Article : Google Scholar : PubMed/NCBI
7	Walton RM, Magnitsky SG, Seiler GS, Poptani H and Wolfe JH: Transplantation and magnetic resonance imaging of canine neural progenitor cell grafts in the postnatal dog brain. J Neuropathol Exp Neurol. 67:954–962. 2008. View Article : Google Scholar : PubMed/NCBI
8	Bulte JW and Kraitchman DL: Iron oxide MR contrast agents for molecular and cellular imaging. NMR Biomed. 17:484–499. 2004. View Article : Google Scholar : PubMed/NCBI
9	Valable S, Barbier EL, Bernaudin M, Roussel S, Segebarth C, Petit E and Rémy C: In vivo MRI tracking of exogenous monocytes/macrophages targeting brain tumors in a rat model of glioma. Neuroimage. 40:973–983. 2008. View Article : Google Scholar
10	Reddy AM, Kwak BK, Shim HJ, Ahn C, Lee HS, Suh YJ and Park ES: In vivo tracking of mesenchymal stem cells labeled with a novel chitosan-coated superparamagnetic iron oxide nanoparticles using 3.0T MRI. J Korean Med Sci. 25:211–219. 2010. View Article : Google Scholar : PubMed/NCBI
11	Heyn C, Ronald JA, Ramadan SS, et al: In vivo MRI of cancer cell fate at the single-cell level in a mouse model of breast cancer metastasis to the brain. Magn Reson Med. 56:1001–1010. 2006. View Article : Google Scholar : PubMed/NCBI
12	Liu G, Xia C, Wang Z, Lv F, Gao F, Gong Q, Song B, Ai H and Gu Z: Magnetic resonance imaging probes for labeling of chondrocyte cells. J Mater Sci Mater Med. 22:601–606. 2011. View Article : Google Scholar : PubMed/NCBI
13	Benda P, Lightbody J, Sato G, Levine L and Sweet W: Differentiated rat glial cell strain in tissue culture. Science. 161:370–371. 1968. View Article : Google Scholar : PubMed/NCBI
14	Vince GH, Bendszus M, Schweitzer T, Goldbrunner RH, Hildebrandt S, Tilgner J, Klein R, Solymosi L, Christian Tonn J and Roosen K: Spontaneous regression of experimental gliomas--an immunohistochemical and MRI study of the C6 glioma spheroid implantation model. Exp Neurol. 190:478–485. 2004. View Article : Google Scholar : PubMed/NCBI
15	Sibenaller ZA, Etame AB, Ali MM, Barua M, Braun TA, Casavant TL and Ryken TC: Genetic characterization of commonly used glioma cell lines in the rat animal model system. Neurosurg Focus. 19:E12005. View Article : Google Scholar : PubMed/NCBI
16	Doblas S, He T, Saunders D, Pearson J, Hoyle J, Smith N, Lerner M and Towner RA: Glioma morphology and tumor-induced vascular alterations revealed in seven rodent glioma models by in vivo magnetic resonance imaging and angiography. J Magn Reson Imaging. 32:267–275. 2010. View Article : Google Scholar
17	Corot C, Robert P, Idée JM and Port M: Recent advances in iron oxide nanocrystal technology for medical imaging. Adv Drug Deliv Rev. 58:1471–1504. 2006. View Article : Google Scholar : PubMed/NCBI
18	de Vries IJ, Lesterhuis WJ, Barentsz JO, et al: Magnetic resonance tracking of dendritic cells in melanoma patients for monitoring of cellular therapy. Nat Biotechnol. 23:1407–1413. 2005.PubMed/NCBI
19	Rice HE, Hsu EW, Sheng H, Evenson DA, Freemerman AJ, et al: Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice. AJR Am J Roentgenol. 188:1101–1108. 2007. View Article : Google Scholar : PubMed/NCBI
20	Fleige G, Nolte C, Synowitz M, Seeberger F, Kettenmann H and Zimmer C: Magnetic labeling of activated microglia in experimental gliomas. Neoplasia. 3:489–499. 2001. View Article : Google Scholar : PubMed/NCBI
21	Meng XX, Wan JQ, Jing M, Zhao SG, Cai W and Liu EZ: Specific targeting of gliomas with multifunctional superparamagnetic iron oxide nanoparticle optical and magnetic resonance imaging contrast agents. Acta Pharmacol Sin. 28:2019–2026. 2007. View Article : Google Scholar
22	Arbab AS, Bashaw LA, Miller BR, Jordan EK, Lewis BK, Kalish H and Frank JA: Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging. Radiology. 229:838–846. 2003. View Article : Google Scholar : PubMed/NCBI
23	Schäfer R, Kehlbach R, Wiskirchen J, Bantleon R, Pintaske J, Brehm BR, Gerber A, Wolburg H, Claussen CD and Northoff H: Transferrin receptor upregulation: in vitro labeling of rat mesenchymal stem cells with superparamagnetic iron oxide. Radiology. 244:514–523. 2007.PubMed/NCBI
24	Walczak P, Kedziorek DA, Gilad AA, Barnett BP and Bulte JW: Applicability and limitations of MR tracking of neural stem cells with asymmetric cell division and rapid turnover: the case of the shiverer dysmyelinated mouse brain. Magn Reson Med. 58:261–269. 2007. View Article : Google Scholar : PubMed/NCBI
25	Gilad AA, Winnard PT Jr, van Zijl PC and Bulte JW: Developing MR reporter genes: promises and pitfalls. NMR Biomed. 20:275–290. 2007. View Article : Google Scholar : PubMed/NCBI
26	Gilad AA, McMahon MT, Walczak P, Winnard PT Jr, Raman V, van Laarhoven HW, Skoglund CM, Bulte JW and van Zijl PC: Artificial reporter gene providing MRI contrast based on proton exchange. Nat Biotechnol. 25:217–219. 2007. View Article : Google Scholar : PubMed/NCBI