microRNA expression profiling in multidrug resistance of the 5‑Fu‑induced SGC‑7901 human gastric cancer cell line

Wang,Yan; Gu,Xiaodong; Li,Zhenyang; Xiang,Jianbin; Jiang,Jianhai; Chen,Zongyou

doi:10.3892/mmr.2013.1384

microRNA expression profiling in multidrug resistance of the 5‑Fu‑induced SGC‑7901 human gastric cancer cell line

Authors:
- Yan Wang
- Xiaodong Gu
- Zhenyang Li
- Jianbin Xiang
- Jianhai Jiang
- Zongyou Chen
View Affiliations

Affiliations: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Key Laboratory of Glycoconjugate Research, Ministry of Public Health and Gene Research Center, Shanghai Medical College of Fudan University, Shanghai 200032, P.R. China
Published online on: March 20, 2013 https://doi.org/10.3892/mmr.2013.1384
Pages: 1506-1510

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The present study aimed to identify microRNA (miRNA) expression profiles associated with multidrug resistance (MDR) in gastric carcinoma. A 5‑ﬂuorouracil (5‑Fu)‑induced MDR gastric cancer cell line was selected and miRNA expression profiling of the cell line was conducted following exposure to 5‑Fu. The miRNA expression profiles between the parental and resistant gastric cancer cells were analyzed by Human miRNA OneArray® v3 and the results were confirmed by quantitative real‑time RT‑PCR. The expression of 9 miRNAs (miR‑10b, ‑22, ‑31, ‑133b, ‑190, ‑501, ‑615, ‑501‑5p and ‑615‑5p) was upregulated while the expression of 18 additional miRNAs (miR‑32, ‑197, ‑210, ‑766, ‑1229, ‑1238, ‑3131, ‑3149, ‑1224‑3p, ‑3162‑3p, ‑532, ‑877, ‑4701‑5p, ‑5096, ‑4728‑3p, ‑1273d, ‑486‑3p and‑4763‑3p) was downregulated in the SGC‑7901/5‑Fu cell line compared with its parental cell line. The results indicate that miRNA expression correlates with MDR in gastric cancer and may serve as biomolecular targets for MDR elimination.

View Figures

View References

1	Zhao P, Dai M, Chen W and Li N: Cancer trends in China. Jpn J Clin Oncol. 40:281–285. 2010. View Article : Google Scholar
2	Jemal A, Siegel R, Ward E, et al: Cancer statistics, 2008. CA Cancer J Clin. 58:71–96. 2008. View Article : Google Scholar
3	de Bree E, Charalampakis V, Melissas J and Tsiftsis DD: The extent of lymph node dissection for gastric cancer: a critical appraisal. J Surg Oncol. 102:552–562. 2010.
4	Lagos-Quintana M, Rauhut R, Lendeckel W and Tuschl T: Identification of novel genes coding for small expressed RNAs. Science. 294:853–858. 2001. View Article : Google Scholar : PubMed/NCBI
5	Cheng AM, Byrom MW, Shelton J and Ford LP: Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis. Nucleic Acids Res. 33:1290–1297. 2005. View Article : Google Scholar : PubMed/NCBI
6	Blower PE, Chung JH, Verducci JS, et al: MicroRNAs modulate the chemosensitivity of tumor cells. Mol Cancer Ther. 7:1–9. 2008. View Article : Google Scholar : PubMed/NCBI
7	Zheng T, Wang J, Chen X and Liu L: Role of microRNA in anticancer drug resistance. Int J Cancer. 126:2–10. 2010. View Article : Google Scholar : PubMed/NCBI
8	Sarkar FH, Li Y, Wang Z, Kong D and Ali S: Implication of microRNAs in drug resistance for designing novel cancer therapy. Drug Resist Updat. 13:57–66. 2010. View Article : Google Scholar : PubMed/NCBI
9	Rossi L, Bonmassar E and Faraoni I: Modification of miR gene expression pattern in human colon cancer cells following exposure to 5-fluorouracil in vitro. Pharmacol Res. 56:248–253. 2007. View Article : Google Scholar : PubMed/NCBI
10	Nakajima G, Hayashi K, Xi Y, et al: Non-coding microRNAs hsa-let-7g and hsa-miR-181b are associated with chemoresponse to S-1 in colon cancer. Cancer Genomics Proteomics. 3:317–324. 2006.PubMed/NCBI
11	Rosenfeld N, Aharonov R, Meiri E, et al: MicroRNAs accurately identify cancer tissue origin. Nat Biotechnol. 26:462–469. 2008. View Article : Google Scholar : PubMed/NCBI
12	Schmittgen TD, Lee EJ, Jiang J, et al: Real-time PCR quantification of precursor and mature microRNA. Methods. 44:31–38. 2008. View Article : Google Scholar : PubMed/NCBI
13	Luqmani YA: Mechanisms of drug resistance in cancer chemotherapy. Med Princ Pract. 14(Suppl 1): 35–48. 2005. View Article : Google Scholar
14	Ganguly A, Banerjee K, Chakraborty P, et al: Overcoming multidrug resistance (MDR) in cancer in vitro and in vivo by a quinoline derivative. Biomed Pharmacother. 65:387–394. 2011. View Article : Google Scholar : PubMed/NCBI
15	Gottesman MM and Pastan I: The multidrug transporter, a double-edged sword. J Biol Chem. 263:1263–1266. 1988.PubMed/NCBI
16	Moscow JA and Cowan KH: Multidrug resistance. J Natl Cancer Inst. 80:14–20. 1988. View Article : Google Scholar : PubMed/NCBI
17	Watson MB, Lind MJ and Cawkwell L: Establishment of in-vitro models of chemotherapy resistance. Anticancer Drugs. 18:749–754. 2007. View Article : Google Scholar : PubMed/NCBI
18	Singh R and Lillard JW Jr: Nanoparticle-based targeted drug delivery. Exp Mol Pathol. 86:215–223. 2009. View Article : Google Scholar : PubMed/NCBI
19	Hammond SM, Bernstein E, Beach D and Hannon GJ: An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature. 404:293–296. 2000. View Article : Google Scholar : PubMed/NCBI
20	Fujita Y, Kojima K, Hamada N, et al: Effects of miR-34a on cell growth and chemoresistance in prostate cancer PC3 cells. Biochem Biophys Res Commun. 377:114–119. 2008. View Article : Google Scholar : PubMed/NCBI
21	Northcott PA, Fernandez-L A, Hagan JP, et al: The miR-17/92 polycistron is up-regulated in sonic hedgehog-driven medulloblastomas and induced by N-myc in sonic hedgehog-treated cerebellar neural precursors. Cancer Res. 69:3249–3255. 2009. View Article : Google Scholar : PubMed/NCBI
22	Li Y, Li W, Yang Y, et al: MicroRNA-21 targets LRRFIP1 and contributes to VM-26 resistance in glioblastoma multiforme. Brain Res. 1286:13–18. 2009. View Article : Google Scholar : PubMed/NCBI
23	Xia L, Zhang D, Du R, et al: miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells. Int J Cancer. 123:372–379. 2008. View Article : Google Scholar : PubMed/NCBI
24	Ma L, Teruya-Feldstein J and Weinberg RA: Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature. 449:682–688. 2007. View Article : Google Scholar : PubMed/NCBI
25	Gee HE, Camps C, Buffa FM, et al: MicroRNA-10b and breast cancer metastasis. Nature. 455:E8–E9. 2008. View Article : Google Scholar : PubMed/NCBI
26	Li G, Wu Z, Peng Y, et al: MicroRNA-10b induced by Epstein-Barr virus-encoded latent membrane protein-1 promotes the metastasis of human nasopharyngeal carcinoma cells. Cancer Lett. 299:29–36. 2010. View Article : Google Scholar : PubMed/NCBI
27	Kakarala M, Brenner DE, Korkaya H, et al: Targeting breast stem cells with the cancer preventive compounds curcumin and piperine. Breast Cancer Res Treat. 122:777–785. 2010. View Article : Google Scholar : PubMed/NCBI