The mechanism through which octreotide inhibits hepatic stellate cell activity

Wang,Jingjing; Wang,Lin; Song,Guanhua; Han,Bo

doi:10.3892/mmr.2013.1385

The mechanism through which octreotide inhibits hepatic stellate cell activity

Authors:
- Jingjing Wang
- Lin Wang
- Guanhua Song
- Bo Han
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Affiliations: Clinical Test Department of Shandong Qianfoshan Hospital, Jinan, Shandong 250014, P.R. China, Department of Pathology, Shandong University Medical School, Jinan, Shandong 250012, P.R. China, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
Published online on: March 20, 2013 https://doi.org/10.3892/mmr.2013.1385
Pages: 1559-1564

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Abstract

Hepatic stellate cells (HSCs) are important in the development of liver fibrosis and in the pathogenesis of portal hypertension. Octreotide, an analogue of somatostatin, has been demonstrated to effectively treat fibrosis and portal hypertension; however, its relative mechanism in HSCs remains unknown. LX‑2, the immortalized HSC line, was used to study the mechanism whereby octreotide functions at different concentrations. Real‑time polymerase chain reaction (PCR) and western blot analysis were used to analyze the expression of fibrosis markers and transcription factors following treatment with octreotide. Soluble secreted endothelin‑1 (ET‑1), collagen I and vascular endothelial growth factor (VEGF) were assessed in the supernatants of cultured cells by enzyme-linked immunosorbent assay (ELISA). In the present study, it was shown that octreotide was able to inhibit the proliferative ability of the LX‑2 cells and decrease the expression of transforming growth factor β (TGF‑β), α‑smooth muscle actin (α‑SMA) and smad‑4a. The transcription factors, including c‑Jun and sp‑1, were downregulated in a dose‑dependent manner following treatment with octreotide. The levels of ET‑1 and collagen I in the supernatant decreased significantly in contrast with the normal levels, whereas the levels of VEGF in the LX‑2 cells and the supernatant increased at a high octreotide concentration (10‑5 nM). Octreotide may exert its effects on ET‑1 or other targeting genes in HSCs through the downregulation of c‑Jun and specificity protein 1 (sp‑1), and the increased levels of VEGF may be the reason for the side effects observed at high concentrations of octreotide.

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