Effects of matrine on oval cell‑mediated liver regeneration and expression of RBP‑Jκ and HES1

Yang,Zhi‑Yun; Wang,Li; Hou,Yi‑Xin; Wang,Xian‑Bo

doi:10.3892/mmr.2013.1398

Effects of matrine on oval cell‑mediated liver regeneration and expression of RBP‑Jκ and HES1

Authors:
- Zhi‑Yun Yang
- Li Wang
- Yi‑Xin Hou
- Xian‑Bo Wang
View Affiliations

Affiliations: Department of Traditional Chinese Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, P.R. China
Published online on: March 27, 2013 https://doi.org/10.3892/mmr.2013.1398
Pages: 1533-1538

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

In the present study a rat model of oval cell‑mediated liver regeneration was constructed to examine the molecular mechanisms of matrine in oval cell‑mediated liver regeneration and the effects of matrine on hepatic function and the expression of OV6 protein and recombination signal sequence‑binding protein Jκ (RBP‑Jκ) and HES1 mRNA. A total of 48 Sprague Dawley rats were equally and randomly assigned to two groups. The model group underwent oval cell‑mediated liver regeneration, whereas the matrine group underwent oval cell‑mediated liver regeneration and received oral gavage of matrine. Expression of OV6 protein was tested by immunohistochemistry and RBP‑Jκ and HES1 mRNA expression was determined by reverse‑transcription polymerase chain reaction. Recovery of hepatic function was faster in the matrine group compared with the model (P<0.05). OV6 protein, RBP‑Jκ and HES1 mRNA expression levels were lower in the matrine than the model group (P<0.05). Matrine promotes oval cell‑mediated liver regeneration through downregulation of the RBP‑Jκ‑HES1 signaling pathway.

View Figures

View References

1	Kuhlmann WD and Peschke P: Hepatic progenitor cells, stem cells and AFP expression in models of liver injury. Int J Exp Pathol. 87:343–359. 2006. View Article : Google Scholar : PubMed/NCBI
2	Libbrecht L and Roskams T: Hepatic progenitor cells in human liver diseases. Semin Cell Dev Biol. 13:389–396. 2002. View Article : Google Scholar : PubMed/NCBI
3	Santoni-Rugiu E, Jelnes P, Thorgeirsson SS and Bisgaard HC: Progenitor cells in liver regeneration: molecular responses controlling their activation and expansion. APMIS. 113:876–902. 2005. View Article : Google Scholar : PubMed/NCBI
4	Lee JS, Heo J, Libbrecht L, et al: A novel prognostic subtype of human hepatocellular carcinoma derived from hepatic progenitor cells. Nat Med. 12:410–416. 2006. View Article : Google Scholar : PubMed/NCBI
5	Shupe T and Petersen BE: Evidence regarding a stem cell origin of hepatocellular carcinoma. Stem Cell Rev. 1:261–264. 2005. View Article : Google Scholar : PubMed/NCBI
6	Li Y, Wang B, Zhou C and Bi Y: Matrine induces apoptosis in angiotensin II-stimulated hyperplasia of cardiac fibroblasts: effects on Bcl-2/Bax expression and caspase-3 activation. Basic Clin Pharmacol Toxicol. 101:1–8. 2007. View Article : Google Scholar : PubMed/NCBI
7	Cheng H, Xia B, Zhang L, et al: Matrine improves 2,4,6-trinitrobenzene sulfonic acid-induced colitis in mice. Pharmacol Res. 53:202–208. 2006. View Article : Google Scholar : PubMed/NCBI
8	Liu J, Zhu M, Shi R and Yang M: Radix Sophorae flavescentis for chronic hepatitis B: a systematic review of randomized trials. Am J Chin Med. 31:337–354. 2003. View Article : Google Scholar
9	Long Y, Lin XT, Zeng KL and Zhang L: Efficacy of intramuscular matrine in the treatment of chronic hepatitis B. Hepatobiliary Pancreat Dis Int. 3:69–72. 2004.PubMed/NCBI
10	Zhang JP, Zhang M, Zhou JP, et al: Antifibrotic effects of matrine on in vitro and in vivo models of liver fibrosis in rats. Acta Pharmacol Sin. 22:183–186. 2001.PubMed/NCBI
11	Ai J, Gao HH, He SZ, Wang L, Luo DL and Yang BF: Effects of matrine, artemisinin, tetrandrine on cytosolic [Ca²⁺]_i in guinea pig ventricular myocytes. Acta Pharmacol Sin. 22:512–515. 2001.PubMed/NCBI
12	Baldi A, de Falco M, de Luca L, et al: Characterization of tissue-specific expression of Notch-1 in human tissues. Biol Cell. 96:303–311. 2004. View Article : Google Scholar : PubMed/NCBI
13	Fausto N and Campbell JS: The role of hepatocytes and oval cells in liver regeneration and repopulation. Mech Dev. 120:117–130. 2003. View Article : Google Scholar : PubMed/NCBI
14	Sautin YY, Jorgensen M, Petersen BE, Saulnier-Blache JS, Crawford JM and Svetlov SI: Hepatic oval (stem) cell expression of endothelial differentiation gene receptors for lysophosphatidic acid in mouse chronic liver injury. J Hematother Stem Cell Res. 11:643–649. 2002. View Article : Google Scholar : PubMed/NCBI
15	Fiegel HC, Havers J, Kneser U, et al: Influence of flow conditions and matrix coatings on growth and differentiation of three-dimensionally cultured rat hepatocytes. Tissue Eng. 10:165–174. 2004. View Article : Google Scholar : PubMed/NCBI
16	Leite AR, Correa-Giannella ML, Dagli ML, et al: Fibronectin and laminin induce expession of islet cell markers in hepatic oval cells in culture. Cell Tissue Res. 327:529–537. 2007. View Article : Google Scholar : PubMed/NCBI
17	Hansson EM, Lendahl U and Chapman G: Notch signaling in development and disease. Semin Cancer Biol. 14:320–328. 2004. View Article : Google Scholar : PubMed/NCBI
18	Stoekhausen MT, Kristofersen K and Poulsen HS: The functional role of Notch signaling in human gliomas. Neuro Oncol. 12:199–211. 2010. View Article : Google Scholar : PubMed/NCBI
19	Kobayashi T and Kageyama R: Hesl regulates embryonic stem cell diferentiation by suppressing Notch signaling. Genes Cells. 15:689–698. 2010. View Article : Google Scholar : PubMed/NCBI
20	Murtaugh LC, Stanger BZ, Kwan KM and Melton DA: Notch sin signaling controls multiple steps of pancreatic differentiation. Proc Natl Acad Sci USA. 100:14920–14925. 2003. View Article : Google Scholar : PubMed/NCBI
21	Okumoto K, Saito T, Hattori E, et al: Differentiation of bone marrow cells into cells that express liver-specific genes in vitro implication of the Notch signals in differentiation. Biochem Biophys Res Commun. 304:691–695. 2003. View Article : Google Scholar : PubMed/NCBI
22	Schwartz RE, Reyes M, Koodie L, et al: Multipotent adult progen itor cells from bone marrow differentiate into functional hepatocyte-like cells. J Clin Invest. 109:1291–1302. 2002. View Article : Google Scholar : PubMed/NCBI