Recombinant Escherichia coli Trx-JZTX-III represses the proliferation of mouse hepatocellular carcinoma cells through induction of cell cycle arrest

  • Authors:
    • Mei-Na Sun
    • Xue-Jiao Zhao
    • Han-Dong Zhao
    • Wei-Guang Zhang
    • Feng-Lan Li
    • Ming-Zi Chen
    • Hui Li
    • Guangchao Li
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  • Published online on: April 16, 2013     https://doi.org/10.3892/mmr.2013.1432
  • Pages: 1800-1804
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Abstract

The aim of the present study was to investigate the effects of recombinant Escherichia coli (E. coli) Trx-jingzhaotoxin (JZTX)-III on cell growth in the mouse hepatocellular carcinoma (HCC) cell line Hepa1-6. The JZTX-III gene sequence was synthesized and cloned into the pET-32a(+) vector to construct the recombinant fusion protein Trx-JZTX-III, which was subsequently purified. Hepa1-6 cells were treated with 0 to 1,000-µg/ml concentrations of Trx-JZTX-III; this was demonstrated to affect cell viability, as determined by the 3-(4,5-dimethylthiazol‑2-yl)-2,5-diphenyltetra-zolium bromide (MTT) assay. The expression of the proliferating cell nuclear antigen (PCNA) protein was investigated using western blot analysis. A colony formation assay was used to determine Hepa1-6 cell proliferation, and the migration ability of cells was determined using a wound‑healing assay. Additionally, flow cytometry was employed to observe changes in the cell cycle. The MTT assay and quantification of PCNA expression indicated that recombinant E. coli Trx-JZTX-III significantly repressed the proliferation of Hepa1-6 cells. Colony formation and the migration of malignant cells was inhibited following treatment with recombinant E. coli Trx-JZTX-III. Flow cytometry showed that recombinant E. coli Trx-JZTX-III induced G0/G1 cell cycle arrest. In conclusion, recombinant E. coli Trx-JZTX-III functions as a tumor suppressor drug in mouse HCC and its underlying mechanism may involve the induction of G0/G1 cell cycle arrest.
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June 2013
Volume 7 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Sun M, Zhao X, Zhao H, Zhang W, Li F, Chen M, Li H and Li G: Recombinant Escherichia coli Trx-JZTX-III represses the proliferation of mouse hepatocellular carcinoma cells through induction of cell cycle arrest. Mol Med Rep 7: 1800-1804, 2013.
APA
Sun, M., Zhao, X., Zhao, H., Zhang, W., Li, F., Chen, M. ... Li, G. (2013). Recombinant Escherichia coli Trx-JZTX-III represses the proliferation of mouse hepatocellular carcinoma cells through induction of cell cycle arrest. Molecular Medicine Reports, 7, 1800-1804. https://doi.org/10.3892/mmr.2013.1432
MLA
Sun, M., Zhao, X., Zhao, H., Zhang, W., Li, F., Chen, M., Li, H., Li, G."Recombinant Escherichia coli Trx-JZTX-III represses the proliferation of mouse hepatocellular carcinoma cells through induction of cell cycle arrest". Molecular Medicine Reports 7.6 (2013): 1800-1804.
Chicago
Sun, M., Zhao, X., Zhao, H., Zhang, W., Li, F., Chen, M., Li, H., Li, G."Recombinant Escherichia coli Trx-JZTX-III represses the proliferation of mouse hepatocellular carcinoma cells through induction of cell cycle arrest". Molecular Medicine Reports 7, no. 6 (2013): 1800-1804. https://doi.org/10.3892/mmr.2013.1432