Ketamine attenuates the lipopolysaccharide-induced inflammatory response in cultured N2a cells

Yang,Chun; Jiang,Ri-Yue; Shen,Jiang; Hong,Tao; Liu,Ning; Ding,Liang-Cai; Wang,Da-Ming; Chen,Lu-Jun; Xu,Bin; Zhu,Bin

doi:10.3892/mmr.2013.1465

Ketamine attenuates the lipopolysaccharide-induced inflammatory response in cultured N2a cells

Authors:
- Chun Yang
- Ri-Yue Jiang
- Jiang Shen
- Tao Hong
- Ning Liu
- Liang-Cai Ding
- Da-Ming Wang
- Lu-Jun Chen
- Bin Xu
- Bin Zhu
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Affiliations: Department of Anesthesiology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China, Department of Radiotherapy, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China, Department of Critical Care Medicine, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China, Department of Comprehensive Laboratory, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China, Department of Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China
Published online on: May 8, 2013 https://doi.org/10.3892/mmr.2013.1465
Pages: 217-220

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Abstract

The use of ketamine is recommended in patients with sepsis undergoing surgery due to its anti-inflammatory effects. However, a paucity of data exists with regard to the anti-inflammatory effects of ketamine in the central nervous system. Therefore, the present study aimed to investigate the effect of ketamine on lipopolysaccharide (LPS)‑induced inflammatory responses in cultured Neuro2a (N2a) cells and to elucidate its potential mechanism of action. N2a cells were randomly divided into the following 3 groups (n=6): The DMEM culture solution administration alone group, the 0.5 µmol/l LPS administration alone group and the 1 µmol/l ketamine plus 0.5 µmol/l LPS administration group. The expression levels of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, nuclear factor (NF)-κB and inducible nitric oxide synthase (iNOS) were determined. LPS-treated N2a cells exhibited a significant increase in the expression levels of IL-1β, IL-6, TNF-α, NF-κB and iNOS, while the administration of ketamine eliminated the LPS-induced production of IL-1β, IL-6, TNF-α, NF-κB and iNOS. Based on our data, we hypothesized that the anti-inflammatory effect exerted by ketamine on N2a cells was potentially due to the inhibition of NF-κB and iNOS.

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