Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach

  • Authors:
    • Jin-Hee Hwang
    • Jin-Ju Oh
    • Tao Wang
    • Yong-Cheng Jin
    • Jae-Sung Lee
    • Jong-Ryeol Choi
    • Kyu-Sup Lee
    • Jong-Kil Joo
    • Hong-Gu Lee
  • View Affiliations

  • Published online on: May 10, 2013     https://doi.org/10.3892/mmr.2013.1469
  • Pages: 183-194
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Abstract

Endometriosis is a gynecological disease defined as the presence of endometrial tissue outside the uterine cavity, which is caused by various factors. Proteomic analysis of two sets of eutopic endometrial cells collected from the menstrual blood of females with (n=6; n=3) or without (n=6; n=3) endometriosis was performed to identify novel potential biomarkers for endometriosis. The data revealed that samples from endometriosis patients had stem cell characteristics, as they had higher mRNA expression levels of octamer-binding transcription factor 4 (Oct-4), C-X-C chemokine receptor type 4 (CXCR4), SRY-box containing gene 2 (SOX2) and mesenchymal-epithelial transition factor (MET) compared with that of the normal controls. Three proteins, collapsin response mediator protein 2 (CRMP2), ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) and myosin regulatory light polypeptide 9 (MYL9), were simultaneously identified from the two sets of samples from females with or without endometriosis by two-dimensional electrophoresis (2-DE). A difference in CRMP2 expression was confirmed with western blotting. Taken together, the results suggest that CRMP2 plays a role in the pathogenesis of endometriosis.
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July 2013
Volume 8 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Hwang J, Oh J, Wang T, Jin Y, Lee J, Choi J, Lee K, Joo J and Lee H: Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach. Mol Med Rep 8: 183-194, 2013
APA
Hwang, J., Oh, J., Wang, T., Jin, Y., Lee, J., Choi, J. ... Lee, H. (2013). Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach. Molecular Medicine Reports, 8, 183-194. https://doi.org/10.3892/mmr.2013.1469
MLA
Hwang, J., Oh, J., Wang, T., Jin, Y., Lee, J., Choi, J., Lee, K., Joo, J., Lee, H."Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach". Molecular Medicine Reports 8.1 (2013): 183-194.
Chicago
Hwang, J., Oh, J., Wang, T., Jin, Y., Lee, J., Choi, J., Lee, K., Joo, J., Lee, H."Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach". Molecular Medicine Reports 8, no. 1 (2013): 183-194. https://doi.org/10.3892/mmr.2013.1469