The effect of MAPK inhibitors and ROS modulators on cell growth and death of H2O2‑treated HeLa cells

  • Authors:
    • Woo Hyun Park
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  • Published online on: June 25, 2013     https://doi.org/10.3892/mmr.2013.1551
  • Pages: 557-564
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Abstract

Reactive oxygen species (ROS) influence the signaling of mitogen‑activated protein kinases (MAPKs) involved in cell survival and death. In the present study, the toxicological effect of hydrogen peroxide (H2O2) on HeLa cervical cancer cells was evaluated following treatment with MAPK inhibitors [MAP kinase or ERK kinase (MEK), c‑Jun N‑terminal kinase (JNK) or p38], N‑acetyl cysteine (NAC) and propyl gallate (PG) (well‑known antioxidants), or L‑buthionine sulfoximine [BSO; an inhibitor of glutathione (GSH) synthesis]. Treatment with 100 µM H2O2 inhibited the growth of HeLa cells and induced cell death, which was accompanied by loss of the mitochondrial membrane potential (MMP; ΔΨm). H2O2 did not induce any specific phase arrests of the cell cycle. ROS levels increased, while GSH levels decreased in H2O2‑treated HeLa cells after 1 and 24 h of treatment. The MAPK inhibitors enhanced H2O2‑induced HeLa cell death, while only p38 inhibitor increased ROS levels. Both NAC and PG attenuated H2O2‑induced HeLa cell growth inhibition and death together with the suppression of ROS levels. BSO increased ROS levels in H2O2‑treated HeLa cells without increasing cell death. The levels of MMP (ΔΨm) loss and GSH depletion were not closely associated with the levels of apoptosis in HeLa cells treated with the MAPK inhibitors, NAC, PG or BSO, in the presence of H2O2. In conclusion, H2O2 induced HeLa cell growth inhibition and death. MAPK inhibitors generally enhanced H2O2‑induced HeLa cell death. In particular, p38 inhibitor increased ROS levels in H2O2‑treated HeLa cells, while NAC and PG attenuated H2O2‑induced HeLa cell death by suppressing ROS levels.
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August 2013
Volume 8 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Park WH: The effect of MAPK inhibitors and ROS modulators on cell growth and death of H2O2‑treated HeLa cells. Mol Med Rep 8: 557-564, 2013.
APA
Park, W.H. (2013). The effect of MAPK inhibitors and ROS modulators on cell growth and death of H2O2‑treated HeLa cells. Molecular Medicine Reports, 8, 557-564. https://doi.org/10.3892/mmr.2013.1551
MLA
Park, W. H."The effect of MAPK inhibitors and ROS modulators on cell growth and death of H2O2‑treated HeLa cells". Molecular Medicine Reports 8.2 (2013): 557-564.
Chicago
Park, W. H."The effect of MAPK inhibitors and ROS modulators on cell growth and death of H2O2‑treated HeLa cells". Molecular Medicine Reports 8, no. 2 (2013): 557-564. https://doi.org/10.3892/mmr.2013.1551