Vascular endothelial growth factor ‑634G/C polymorphism is associated with increased breast cancer risk and aggressiveness

Sa‑Nguanraksa,Doonyapat; Chuangsuwanich,Tuenjai; Pongpruttipan,Tawatchai; Kummalue,Tanawan; Rojananin,Supakorn; Ratanawichhitrasin,Adune; Prasarttong‑Osoth,Poramaporn; Chuthatisith,Suebwong; Pisarnturakit,Pongthep; Aeumrithaicharoenchok,Waraporn; Rushatamukayanunt,Pradit; Lohsiriwat,Visnu; Boonsripitayanon,Mongkol; Malasit,Prida; O‑Charoenrat,Pornchai

doi:10.3892/mmr.2013.1607

Vascular endothelial growth factor ‑634G/C polymorphism is associated with increased breast cancer risk and aggressiveness

Authors:
- Doonyapat Sa‑Nguanraksa
- Tuenjai Chuangsuwanich
- Tawatchai Pongpruttipan
- Tanawan Kummalue
- Supakorn Rojananin
- Adune Ratanawichhitrasin
- Poramaporn Prasarttong‑Osoth
- Suebwong Chuthatisith
- Pongthep Pisarnturakit
- Waraporn Aeumrithaicharoenchok
- Pradit Rushatamukayanunt
- Visnu Lohsiriwat
- Mongkol Boonsripitayanon
- Prida Malasit
- Pornchai O‑Charoenrat
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Affiliations: Division of Head, Neck and Breast Surgery, Department of Surgery, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand, Department of Pathology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand, Department of Clinical Pathology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand, Dengue Hemorrhagic Fever Research Unit, Office for Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Published online on: July 31, 2013 https://doi.org/10.3892/mmr.2013.1607
Pages: 1242-1250

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Abstract

Polymorphisms in the promoter and 5' untranslated region of vascular endothelial growth factor (VEGF) have been associated with VEGF levels. To investigate the role of VEGF polymorphisms in breast cancer, the VEGF ‑2578C/A, ‑1498C/T, ‑1154G/A and ‑634G/C polymorphisms were genotyped in 483 breast cancer patients and 524 healthy controls. VEGF mRNA levels in breast cancer tissue were determined using semi‑quantitative RT‑PCR. The genotypes, ‑634G/C and ‑634C/C, were associated with an increased risk for breast cancer when compared with the ‑634G/G genotype. The VEGF ‑634G/C genotype was associated with tumor size >20 mm, perineural invasion and stage II‑IV. Individuals with ‑634C/C had lower disease‑free survival. Patients with the VEGF ‑634C/C genotype exhibited the highest VEGF mRNA levels. High VEGF mRNA expression correlated with tumor size >20 mm, presence of lymphovascular invasion and axillary nodal metastasis. These observations suggested that VEGF ‑634G/C polymorphisms have a significant role in breast cancer susceptibility and aggressiveness.

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