RACK1 promotes prostate cancer cell proliferation, invasion and metastasis

Shen,Fangrong; Yan,Chunyin; Liu,Ming; Feng,Yahong; Chen,Youguo

doi:10.3892/mmr.2013.1612

RACK1 promotes prostate cancer cell proliferation, invasion and metastasis

Authors:
- Fangrong Shen
- Chunyin Yan
- Ming Liu
- Yahong Feng
- Youguo Chen
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Affiliations: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Obstetrics and Gynecology, Suzhou Kowloon Hospital, Suzhou, Jiangsu 215021, P.R. China, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
Published online on: August 2, 2013 https://doi.org/10.3892/mmr.2013.1612
Pages: 999-1004

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Abstract

The aim of the present study was to investigate the functions of RACK1 and its involvement in mechanisms of prostate cancer (PC) cell proliferation, invasion and metastasis. The proliferation, invasion and metastasis of stably transfected DU145 cells with RACK1 was evaluated in vitro as well as in vivo following the establishment of nude mouse models. The expression of Ki67, RACK1, PTEN and androgen receptor (AR) in PC was detected by immunohistochemical analysis. Our results indicated that RACK1 promotes PC cell proliferation, invasion and metastasis in vitro and in vivo. However, knockdown of RACK1 by siRNA in vitro inhibited PC cell proliferation, migration and invasion. PTEN downregulation and Ki67 upregulation were also altered with the upregulation of RACK1; RACK1 staining was strongly correlated with PTEN downregulation and Ki67 upregulation. These data demonstrated that increased RACK1 expression is important in promoting PC cell proliferation, invasion and metastasis in vitro and in vivo.

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