Determination of the effects of lactoferrin in a preclinical mouse model of experimental colitis

Li,Li; Ren,Fazheng; Yun,Zhanyou; An,Ying; Wang,Caiyun; Yan,Xudong

doi:10.3892/mmr.2013.1632

Determination of the effects of lactoferrin in a preclinical mouse model of experimental colitis

Authors:
- Li Li
- Fazheng Ren
- Zhanyou Yun
- Ying An
- Caiyun Wang
- Xudong Yan
View Affiliations

Affiliations: Technology Center, Inner Mongolia Yili Industrial Group Co., Ltd., Hohhot, Inner Mongolia Autonomous Region 010110, P.R. China, Key Laboratory of Functional Dairy, Co‑constructed by the Ministry of Education and Beijing Municipality, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R. China
Published online on: August 14, 2013 https://doi.org/10.3892/mmr.2013.1632
Pages: 1125-1129

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of this study was to determine the therapeutic efficacy of lactoferrin (Lf) on dextran sulphate sodium (DSS)‑induced experimental colitis in BALB/c mice. Eighty BALB/c mice were randomly divided into 4 groups; the normal, model, apo‑Lf and holo‑Lf groups. Fecal character, fecal occult blood, hematochezia and disease activity index (DAI) were recorded daily. The length of the colon was measured and histological scores were evaluated 28 days post‑treatment. Myeloperoxidase (MPO) activity was also determined and the expression of interleukin‑1β (IL‑1β) and tumor necrosis factor-α (TNF‑α) were measured by quantitative (q)PCR. Lf relieved the inflammatory condition of DSS‑induced experimental colitis in mice. The DAI and histological scores of Lf‑treated mice were lower compared with those of mice in the control group. The length of the colon of Lf‑treated mice was longer compared with that of mice in the control group. Treatment with Lf decreased MPO activity and the expression levels of IL‑1β and TNF‑α. In addition, Lf was found to promote beneficial effects in a mouse model of experimental colitis. Treatment with apo‑Lf was superior to that of holo‑Lf in the mouse model of DSS‑induced experimental colitis. Supplemental therapy with apo‑Lf may provide an important new tool in the clinical management of ulcerative colitis.

View Figures

View References

1	Legrand D, Elass E, Carpentier M and Mazurier J: Lactoferrin: a modulator of immune and inflammatory responses. Cell Mol Life Sci. 62:2549–2559. 2005. View Article : Google Scholar : PubMed/NCBI
2	Valenti P and Antonini G: Lactoferrin: an important host defence against microbial and viral attack. Cell Mol Life Sci. 62:2576–2587. 2005. View Article : Google Scholar : PubMed/NCBI
3	Mogensen SC and Virelizier JL: The interferon-macrophage alliance. Interferon. 8:55–84. 1987.PubMed/NCBI
4	Jiang XL and Cui HF: An analysis of 10218 ulcerative colitis cases in China. World J Gastroenterol. 8:158–161. 2002.PubMed/NCBI
5	Carvalho R and Hyams JS: Diagnosis and management of inflammatory bowel disease in children. Semin Pediatr Surg. 16:164–171. 2007. View Article : Google Scholar
6	Shanahan F: Crohn’s disease. Lancet. 359:62–69. 2002.
7	Togawa J, Nagase H, Tanaka K, et al: Lactoferrin reduces colitis in rats via modulation of the immune system and correction of cytokine imbalance. Am J Physiol Gastrointest Liver Physiol. 283:G187–G195. 2002. View Article : Google Scholar
8	Lu RR: Isolation, purification and bioactive functions of lactoferrin (unpublished PhD thesis). Jiangnan University; 2003, (In Chinese).
9	Hamamoto N, Maemura K, Hirata I, Murano M, Sasaki S and Katsu K: Inhibition of dextran sulphate sodium (DSS)-induced colitis in mice by intracolonically administered antibodies against adhesion molecules (endothelial leucocyte adhesion molecule-1 (ELAM-1) or intercellular adhesion molecule-1 (ICAM-1)). Clin Exp Immunol. 117:462–468. 1999. View Article : Google Scholar
10	Cooper HS, Murthy SN, Shah RS and Sedergran DJ: Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest. 69:238–249. 1993.PubMed/NCBI
11	Fabia R, Ar’Rajab A, Johansson ML, et al: The effect of exogenous administration of Lactobacillus reuteri R2LC and oat fiber on acetic acid-induced colitis in the rat. Scand J Gastroenterol. 28:155–162. 1993.PubMed/NCBI
12	Egger B, Bajaj-Elliott M, MacDonald TT, Inglin R, Eysselein VE and Büchler MW: Characterisation of acute murine dextran sodium sulphate colitis: cytokine profile and dose dependency. Digestion. 62:240–248. 2000. View Article : Google Scholar : PubMed/NCBI
13	Zhang SZ, Zhao XH and Zhang DC: Cellular and molecular immunopathogenesis of ulcerative colitis. Cell Mol Immunol. 3:35–40. 2006.PubMed/NCBI
14	Yan Y, Kolachala V, Dalmasso G, et al: Temporal and spatial analysis of clinical and molecular parameters in dextran sodium sulfate induced colitis. PLoS One. 4:e60732009. View Article : Google Scholar : PubMed/NCBI
15	Beagley KW and Elson CO: Cells and cytokines in mucosal immunity and inflammation. Gastroenterol Clin North Am. 21:347–366. 1992.PubMed/NCBI
16	Ishiguro Y: Mucosal proinflammatory cytokine production correlates with endoscopic activity of ulcerative colitis. J Gastroenterol. 34:66–74. 1999. View Article : Google Scholar : PubMed/NCBI
17	Wang QY, Chen CL, Wang JD, Lai ZS, Ma Q and Zhang YL: Expression of pro-inflammation cytokines and activation of nuclear factor kappab in the intestinal mucosa of mice with ulcerative colitis. Di Yi Jun Yi Da Xue Xue Bao. 23:1202–1205. 2003.(In Chinese).
18	Rachmilewitz D, Karmeli F, Shteingart S, Lee J, Takabayashi K and Raz E: Immunostimulatory oligonucleotides inhibit colonic proinflammatory cytokine production in ulcerative colitis. Inflamm Bowel Dis. 12:339–345. 2006. View Article : Google Scholar : PubMed/NCBI
19	Kim WS, Tanaka T, Kumura H and Shimazaki K: Lactoferrin-binding proteins in Bifidobacterium bifidum. Biochem Cell Biol. 80:91–94. 2002. View Article : Google Scholar
20	Tomita M, Wakabayashi H, Yamauchi K, Teraguchi S and Hayasawa H: Bovine lactoferrin and lactoferricin derived from milk: production and applications. Biochem Cell Biol. 80:109–112. 2002. View Article : Google Scholar : PubMed/NCBI
21	Cheng XY and Zhang LL: The relation of fulminant hepatitis with Endotoxin and its receptors and inflammatory cytokines. Jiangxi Medical Journal. 41:114–117. 2006.(In Chinese).