Citrate induces apoptosis of the acute monocytic leukemia U937 cell line through regulation of HIF‑1α signaling

Xu,Xiaowei; Li,Baiwen; Huang,Peijie; Wan,Xinjian; Qin,Youwen; Zhou,Lili; Liu,Huixia; Bai,Haitao; Gao,Yanrong; Wang,Chun; Meng,Xiangjun

doi:10.3892/mmr.2013.1702

Citrate induces apoptosis of the acute monocytic leukemia U937 cell line through regulation of HIF‑1α signaling

Authors:
- Xiaowei Xu
- Baiwen Li
- Peijie Huang
- Xinjian Wan
- Youwen Qin
- Lili Zhou
- Huixia Liu
- Haitao Bai
- Yanrong Gao
- Chun Wang
- Xiangjun Meng
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Affiliations: Department of Hematology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. Chian, Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China, Department of Hematology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. Chian, Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China, Department of Gastroenterology, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, P.R. China
Published online on: September 24, 2013 https://doi.org/10.3892/mmr.2013.1702
Pages: 1379-1384

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Abstract

The present study aimed to investigate the anti‑tumor effect of citrate on acute monocytic leukemia (AML) and its mechanisms. The apoptosis of the AML cell line, U937, was assessed by MTT and Hoechst staining, the expression of Bcl‑2, caspases‑3 and ‑9, hypoxia‑inducible factor 1α (HIF‑1α) and its target gene GLUT‑1, were assayed by western blotting and the role of HIF‑1α was evaluated through siRNA. The results showed that citrate inhibits the expression of Bcl‑2, while it induces the activation of caspases‑3 and ‑9. In addition, citrate induces U937 apoptosis in a dose‑ and time‑dependent manner by regulating the expression of HIF‑1α and its downstream target GLUT‑1. The results suggest that citrate performs an anti‑acute monocytic leukemia action by targeting HIF‑1α signaling and may be a promising clinical approach.

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