Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media

  • Authors:
    • Xiu M. Guan
    • Min Cheng
    • Hong Li
    • Xiao D. Cui
    • Xin Li
    • Yu L. Wang
    • Jin L. Sun
    • Xiao Y. Zhang
  • View Affiliations

  • Published online on: October 9, 2013     https://doi.org/10.3892/mmr.2013.1718
  • Pages: 1722-1728
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Abstract

Ex vivo expansion of endothelial progenitor cells (EPCs) may be a promising strategy to overcome the clinical problem of limited cell numbers. As the culture medium is the key for the cell characteristics, the effects of different culture media on EPCs were investigated in the present study. Rat bone marrow mononuclear cells were cultured in different media, including M-199 media with 20% fetal bovine serum (FBS) and bovine pituitary extract (M1); M-199 media with 10% FBS, 20 ng/ml vascular endothelial growth factor (VEGF) and 10 ng/ml basic fibroblast growth factor (bFGF; M2) or epidermal growth medium (EGM)-2MV media. The cell morphology and biological functions, such as proliferation, adhesion, migration, tube formation and nitric oxide (NO) production were subsequently assayed in vitro. Moreover, endothelial biomarkers and apoptosis were also analyzed. The results showed that endothelial‑like cells appeared in all of the culture systems. First‑passage cells, namely early EPCs, tended to form colonies in M2 and EGM-2MV media but showed a fusiform shape in M1 media. The 3rd or 4th generation EPCs, namely late EPCs, cultured in EGM-2MV media exhibited increased adhesion, migration, tube formation and NO production as compared with EPCs in M1 or M2 media. Furthermore, late EPCs cultured in EGM-2MV expressed higher levels of endothelial cell markers, such as von Willibrand factor (vWF)and CD31, but relatively greater levels of apoptosis were observed. In conclusion, cell culture conditions, for example the medium used, affects the biological properties of bone marrow-derived early and late EPCs.
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December 2013
Volume 8 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Guan XM, Cheng M, Li H, Cui XD, Li X, Wang YL, Sun JL and Zhang XY: Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media. Mol Med Rep 8: 1722-1728, 2013.
APA
Guan, X.M., Cheng, M., Li, H., Cui, X.D., Li, X., Wang, Y.L. ... Zhang, X.Y. (2013). Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media. Molecular Medicine Reports, 8, 1722-1728. https://doi.org/10.3892/mmr.2013.1718
MLA
Guan, X. M., Cheng, M., Li, H., Cui, X. D., Li, X., Wang, Y. L., Sun, J. L., Zhang, X. Y."Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media". Molecular Medicine Reports 8.6 (2013): 1722-1728.
Chicago
Guan, X. M., Cheng, M., Li, H., Cui, X. D., Li, X., Wang, Y. L., Sun, J. L., Zhang, X. Y."Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media". Molecular Medicine Reports 8, no. 6 (2013): 1722-1728. https://doi.org/10.3892/mmr.2013.1718