Brain-derived neurotrophic factor expression in dorsal root ganglia of a lumbar spinal stenosis model in rats

Li,Qinliang; Liu,Yi; Chu,Zhaoming; Chen,Jinchuan; Dai,Fenglei; Zhu,Xiaorong; Hu,Ankang; Yun,Cai

doi:10.3892/mmr.2013.1723

Brain-derived neurotrophic factor expression in dorsal root ganglia of a lumbar spinal stenosis model in rats

Authors:
- Qinliang Li
- Yi Liu
- Zhaoming Chu
- Jinchuan Chen
- Fenglei Dai
- Xiaorong Zhu
- Ankang Hu
- Cai Yun
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Affiliations: Department of Spine, The Affiliated Lianyungang Hospital of Xuzhou Medical College, Lianyungang, Jiangsu 222000, P.R. China, Department of Spine, Nanjing Liuhe District People's Hospital, Nanjing, Jiangsu 210000, P.R. China, Experimental Animal Center, Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China, Department of Orthopaedic, Shijingshan Hospital of Beijing City, Beijing 100043, P.R. China
Published online on: October 11, 2013 https://doi.org/10.3892/mmr.2013.1723
Pages: 1836-1844

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Abstract

This study aimed to investigate the expression of brain-derived neurotrophic factor (BDNF) in dorsal root ganglia (DRG) of a rat model of lumbar spinal stenosis (LSS). Adult male rats were divided into the operation and sham operation groups. The operation group was comprised of the rat models of LSS. Walking distance and BDNF expression levels in DRG were measured in the two groups at different time points. The total BDNF protein levels and positive cell mean optical density (MOD) values in the operation group were significantly higher at each time point compared with that of the sham operation and preoperative control groups (P<0.05). The total BDNF protein levels and MOD values following sport in the operation group were significantly higher compared with those prior to sport (P<0.05). In the sham operation group, BDNF protein levels and MOD values before and after sport at each time point showed no significant differences than those of the operation group (P>0.05). Moreover, BDNF protein levels and MOD values in the operation group indicated a negative correlation with walking distance. The present study demonstrated that the expression of BDNF in rat models of LSS increased with time and was associated with a decrease in walking distance. BDNF was therefore important for the process of intermittent claudication caused by LSS.

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