Anti‑inflammatory and anti‑proliferative effects of Rhus verniciflua Stokes in RAW264.7 cells

  • Authors:
    • Han‑Seok Choi
    • Hye Sook Seo
    • Soon Re Kim
    • Youn Kyung Choi
    • Bo-Hyoung Jang
    • Yong‑Cheol Shin
    • Seong‑Gyu Ko
  • View Affiliations

  • Published online on: November 4, 2013     https://doi.org/10.3892/mmr.2013.1775
  • Pages: 311-315
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Abstract

Inflammatory response is a major defense mechanism against pathogens and chemical or mechanical injury. Rhus verniciflua Stokes (RVS) has traditionally been used as an ingredient in East Asian medicine for the treatment of gastritis, stomach cancer and atherosclerosis. The aim of the current study was to analyze the effect of RVS on LPS‑induced inflammatory responses in the RAW264.7 mouse macrophage cell line. RAW264.7 cells were treated with various concentrations of RVS and LPS at specific time points. WST assay, trypan blue assay and quantification of activated cells revealed that RVS suppressed cell proliferation in a dose‑dependent manner. RVS induced G1 cell cycle arrest, suppressed iNOS and COX‑2 mRNA expression induced by LPS and decreased intracellular ROS levels induced by LPS. In addition, RVS induced PARP and caspase‑3 cleavage suggesting that RVS causes cell death. Results of the present study indicate that RVS may be advantageous in treating inflammatory disease.
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2014-January
Volume 9 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Choi HS, Seo HS, Kim SR, Choi YK, Jang B, Shin YC and Ko SG: Anti‑inflammatory and anti‑proliferative effects of Rhus verniciflua Stokes in RAW264.7 cells. Mol Med Rep 9: 311-315, 2014.
APA
Choi, H., Seo, H.S., Kim, S.R., Choi, Y.K., Jang, B., Shin, Y., & Ko, S. (2014). Anti‑inflammatory and anti‑proliferative effects of Rhus verniciflua Stokes in RAW264.7 cells. Molecular Medicine Reports, 9, 311-315. https://doi.org/10.3892/mmr.2013.1775
MLA
Choi, H., Seo, H. S., Kim, S. R., Choi, Y. K., Jang, B., Shin, Y., Ko, S."Anti‑inflammatory and anti‑proliferative effects of Rhus verniciflua Stokes in RAW264.7 cells". Molecular Medicine Reports 9.1 (2014): 311-315.
Chicago
Choi, H., Seo, H. S., Kim, S. R., Choi, Y. K., Jang, B., Shin, Y., Ko, S."Anti‑inflammatory and anti‑proliferative effects of Rhus verniciflua Stokes in RAW264.7 cells". Molecular Medicine Reports 9, no. 1 (2014): 311-315. https://doi.org/10.3892/mmr.2013.1775