Anti-inflammatory effects of 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone via NF-κB inactivation in lipopolysaccharide-stimulated RAW 264.7 macrophage

Kim,Min Jeong; Lee,Hye Hyeon; Jeong,Jin Woo; Seo,Min Jeong; Kang,Byoung Won; Park,Jeong Uck; Kim,Kyoung-Sook; Cho,Young-Su; Seo,Kwon-Il; Kim,Gi-Young; Kim,Jung-In; Choi,Yung Hyun; Jeong,Yong Kee

doi:10.3892/mmr.2014.1922

Anti-inflammatory effects of 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone via NF-κB inactivation in lipopolysaccharide-stimulated RAW 264.7 macrophage

Authors:
- Min Jeong Kim
- Hye Hyeon Lee
- Jin Woo Jeong
- Min Jeong Seo
- Byoung Won Kang
- Jeong Uck Park
- Kyoung-Sook Kim
- Young-Su Cho
- Kwon-Il Seo
- Gi-Young Kim
- Jung-In Kim
- Yung Hyun Choi
- Yong Kee Jeong
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Affiliations: Department of Biotechnology, Dong-A University, Saha‑gu, Busan 604-714, Republic of Korea, Center for Core Research Facilities, Daegu Gyeongbuk Institute of Science and Technology, Dalseong‑gun, Daegu 711-873, Republic of Korea, Medi-Farm Industrialization Research Center, Dong-A University, Saha‑gu, Busan 604-714, Republic of Korea, Department of Food and Nutrition, Sunchon National University, Sunchon, Jeollanam‑do 540-950, Republic of Korea, Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju-si, Jeju 690-756, Republic of Korea, School of Food and Life Sciences, Inje University, Gimhae, Gyeongsang 621-749, Republic of Korea, Department of Biochemistry, Dongeui University College of Oriental Medicine, Busanjin-gu, Busan 614-052, Republic of Korea
Published online on: January 28, 2014 https://doi.org/10.3892/mmr.2014.1922
Pages: 1197-1203

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Abstract

The anti-inflammatory mechanism of 5‑hydroxy-3,6,7,8,3',4'‑hexamethoxyflavone (5HHMF), a polyhydroxyflavone isolated from the marine algae Hizikia fusiforme, was investigated in RAW 264.7 murine macrophage cells. Western blot and reverse transcriptase PCR analyses indicated that adding 5HHMF to cultured cells significantly reduced the production of nitric oxide and prostaglandin E2 and downregulated inducible nitric oxide synthase (iNOS) and cyclooxygenase‑2 (COX‑2) expression in lipopolysaccharide (LPS)‑stimulated RAW 264.7 cells. In addition, 5HHMF inhibited the release of pro‑inflammatory cytokines, such as tumor necrosis factor‑α and interleukin‑1β, and decreased the transcriptional levels. In particular, 5HHMF significantly inhibited the LPS‑induced nuclear factor‑κB (NF‑κB) translocation from the cytosol to the nucleus, which was associated with the abrogation of inhibitory IκBα degradation and subsequent decreases in nuclear p65 levels. In conclusion, these results suggested that the anti‑inflammatory activities of 5HHMF may be attributed to the inhibition of iNOS, COX‑2 and cytokine expression by attenuating NF‑κB activation via IκBα degradation in macrophages.

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