Anti-inflammatory activity of a methanol extract from Ardisia tinctoria on mouse macrophages and paw edema

Kim,Hui-Seong; Park,Ji-Won; Kwon,Ok-Kyoung; Kim,Jea-Hong; Oh,Sei-Ryang; Lee,Hyeong-Kyu; Bach,Tran   The; Quang,Bui  Hong; Ahn,Kyung-Seop

doi:10.3892/mmr.2014.1941

Anti-inflammatory activity of a methanol extract from Ardisia tinctoria on mouse macrophages and paw edema

Authors:
- Hui-Seong Kim
- Ji-Won Park
- Ok-Kyoung Kwon
- Jea-Hong Kim
- Sei-Ryang Oh
- Hyeong-Kyu Lee
- Tran The Bach
- Bui Hong Quang
- Kyung-Seop Ahn
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Affiliations: Biomolecular Science, University of Science and Technology, Daejeon 305‑806, Republic of Korea, Department of Biotechnology, Korea University, Seoul 136‑713, Republic of Korea, Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea, Department of Botany, Institute of Ecology and Biological Resources,Vietnam Academy of Science and Technology, Hanoi 10307, Vietnam, Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk 363-883, Republic of Korea
Published online on: February 10, 2014 https://doi.org/10.3892/mmr.2014.1941
Pages: 1388-1394

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Abstract

Ardisia tinctoria (AT) is a plant of the Myrsinaceae family. No studies on its anti-inflammatory effects have yet been reported. This study investigated the anti-inflammatory activity of AT. A non-cytotoxic methanol extract of AT inhibited the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), leading to significantly reduced levels of nitric oxide (NO) and prostaglandin E2 (PGE2) and of two proteins regulated by these, interleukin-1β (IL-1β) and IL-6, in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. The thickness of paw edema induced in vivo in mice by carrageenan administration was effectively reduced by the AT extract. Translocation of the nuclear factor-κB (NF-κB) subunit 65 (p65) into the nucleus and phosphorylation of mitogen-activated protein kinase kinase (MEK) and extracellular signal-related kinase (ERK) were inhibited by the AT extract. Our results indicated that a methanol extract of AT downregulates the inflammatory response by blocking phosphorylation of MEK and ERK and activation of NF-κB. To the best of our knowledge, this is the first study of anti-inflammatory effects of an AT extract, and demonstrates its potential in the treatment of inflammatory diseases.

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