Pharmacological evaluation for anticancer and immune activities of a novel polysaccharide isolated from Boletus speciosus Frost

Hou,Yiling; Ding,Xiang; Hou,Wanru; Song,Bo; Wang,Ting; Wang,Fang; Li,Jian; Zeng,Yichun; Zhong,Jie; Xu,Ting; Zhu,Hongqing

doi:10.3892/mmr.2014.1976

Pharmacological evaluation for anticancer and immune activities of a novel polysaccharide isolated from Boletus speciosus Frost

Authors:
- Yiling Hou
- Xiang Ding
- Wanru Hou
- Bo Song
- Ting Wang
- Fang Wang
- Jian Li
- Yichun Zeng
- Jie Zhong
- Ting Xu
- Hongqing Zhu
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Affiliations: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong, Sichuan 637009, P.R. China
Published online on: February 20, 2014 https://doi.org/10.3892/mmr.2014.1976
Pages: 1337-1344

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Abstract

The fungal polysaccharides have been revealed to exhibit a variety of biological activities, including antitumor, immune‑stimulation and antioxidation activities. In the present study, the immune and anticancer activities of a novel polysaccharide, BSF‑A, isolated from Boletus speciosus Frost was investigated. The inhibitory rate of S180 tumors in mice treated with 40 mg/kg BSF‑A reached 62.449%, which was the highest rate from the three doses administered; this may be comparable to mannatide. The antitumor activity of BSF‑A is commonly considered to be a consequence of the stimulation of the cell‑mediated immune response, as it may significantly promote the macrophage cells in the dose range of 100‑400 µg/ml in vitro. The levels of the cytokines, IL‑6, IL‑1β and TNF‑α, and nitric oxide, induced by BSF‑A treatment at varying concentrations in the macrophage cells were similar to the levels in the cells treated with lipopolysaccharide. There was weak expression of the TNF‑α, IL‑6, IL‑1β and inducible nitric oxide synthase mRNA in the untreated macrophages, but this increased significantly in a dose‑dependent manner in the BSF‑A‑treated cells. BSF‑A also had a time‑ and dose‑dependent effect on the growth inhibition of the Hep‑2 cells, with the concentration of 400 µg/ml having the highest inhibitory rate. A quantitative PCR array analysis of the gene expression profiles indicated that BSF‑A had anticancer activities that affected cell apoptosis in the Hep‑2 cells. The results obtained in the present study indicated that the purified polysaccharide of Boletus speciosus Frost is a potential source of natural anticancer substances.

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