Expression of β‑amyloid precursor protein in refractory epilepsy

Sima,Xiutian; Xu,Jianguo; Li,Jinmei; Zhong,Weiying; You,Chao

doi:10.3892/mmr.2014.1977

Expression of β‑amyloid precursor protein in refractory epilepsy

Authors:
- Xiutian Sima
- Jianguo Xu
- Jinmei Li
- Weiying Zhong
- Chao You
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Affiliations: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Neurology, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: February 21, 2014 https://doi.org/10.3892/mmr.2014.1977
Pages: 1242-1248

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Abstract

β‑amyloid precursor protein (β‑APP), also known as Aβ peptide, has a key role in the pathogenesis of Alzheimer's disease, and is also likely to be involved in the development of refractory epilepsy. The mechanism behind the association between β‑APP and refractory epilepsy remains to be elucidated. The aim of the present study was to examine the levels of APP mRNA and β‑APP protein in patients with refractory epilepsy. Tissue samples were obtained from patients with chronic pharmacoresistant epilepsy who underwent surgery. Levels of APP mRNA and β‑APP protein in epileptic temporal lobe and hippocampal tissue were assessed using quantitative polymerase chain reaction, immunohistochemistry and immunofluorescence. The expression levels of protein significantly increased in the temporal cortex and the hippocampus of the patients with epilepsy. β‑APP may thus contribute to the pathogenesis of refractory epilepsy.

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1	Lozsadi DA and Larner AJ: Prevalence and causes of seizures at the time of diagnosis of probable Alzheimer’s disease. Dement Geriatr Cogn Disord. 22:121–124. 2006.
2	Del Vecchio RA, Gold LH, Novick SJ, Wong G and Hyde LA: Increased seizure threshold and severity in young transgenic CRND8 mice. Neurosci Lett. 367:164–167. 2004.PubMed/NCBI
3	Shoham S and Ebstein RP: The distribution of beta-amyloid precursor protein in rat cortex after systemic kainate-induced seizures. Exp Neurol. 147:361–376. 1997. View Article : Google Scholar : PubMed/NCBI
4	Sheng JG, Boop FA, Mrak RE and Griffin WS: Increased neuronal beta-amyloid precursor protein expression in human temporal lobe epilepsy: association with interleukin-1 alpha immunoreactivity. J Neurochem. 63:1872–1879. 1994. View Article : Google Scholar
5	Willoughby DA, Johnson SA, Pasinetti GM, et al: Amyloid precursor protein mRNA encoding the Kunitz protease inhibitor domain is increased by kainic acid-induced seizures in rat hippocampus. Exp Neurol. 118:332–339. 1992. View Article : Google Scholar : PubMed/NCBI
6	Ferreira A, Caceres A and Kosik KS: Intraneuronal compartments of the amyloid precursor protein. J Neurosci. 13:3112–3123. 1993.PubMed/NCBI
7	Wieser HG, Blume WT, Fish D, et al: ILAE Commission Report. Proposal for a new classification of outcome with respect to epileptic seizures following epilepsy surgery. Epilepsia. 42:282–286. 2001. View Article : Google Scholar
8	Radhakrishnan VV, Rao MB, Radhakrishnan K, et al: Pathology of temporal lobe epilepsy: An analysis of 100 consecutive surgical specimens from patients with medically refractory epilepsy. Neurol India. 47:196–201. 1999.PubMed/NCBI
9	Larner AJ: Axonal sprouting and synaptogenesis in temporal lobe epilepsy: possible pathogenetic and therapeutic roles of neurite growth inhibitory factors. Seizure. 4:249–258. 1995. View Article : Google Scholar : PubMed/NCBI
10	Nadler JV: The recurrent mossy fiber pathway of the epileptic brain. Neurochem Res. 28:1649–1658. 2003. View Article : Google Scholar : PubMed/NCBI
11	Yamazaki T, Selkoe DJ and Koo EH: Trafficking of cell surface beta-amyloid precursor protein: retrograde and transcytotic transport in cultured neurons. J Cell Biol. 129:431–442. 1995. View Article : Google Scholar : PubMed/NCBI
12	Kins S, Lauther N, Szodorai A and Beyreuther K: Subcellular trafficking of the amyloid precursor protein gene family and its pathogenic role in Alzheimer’s disease. Neurodegener Dis. 3:218–226. 2006.PubMed/NCBI
13	Smith DH, Uryu K, Saatman KE, Trojanowski JQ and McIntosh TK: Protein accumulation in traumatic brain injury. Neuromolecular Med. 4:59–72. 2003. View Article : Google Scholar : PubMed/NCBI
14	Bramlett HM, Kraydieh S, Green EJ and Dietrich WD: Temporal and regional patterns of axonal damage following traumatic brain injury: a beta-amyloid precursor protein immunocytochemical study in rats. J Neuropathol Exp Neurol. 56:1132–1141. 1997. View Article : Google Scholar : PubMed/NCBI
15	Perez RG, Zheng H, Van der Ploeg LH and Koo EH: The beta-amyloid precursor protein of Alzheimer’s disease enhances neuron viability and modulates neuronal polarity. J Neurosci. 17:9407–9414. 1997.
16	Gordon-Krajcer W and Gajkowska B: Excitotoxicity-induced expression of amyloid precursor protein (beta-APP) in the hippocampus and cortex of rat brain. An electron-microscopy and biochemical study. Folia Neuropathol. 39:163–173. 2001.PubMed/NCBI
17	Zhu JL, Zhu SH and Ren L: Expression of beta-amyloid precursor protein in diffuse axonal injury of rats. Fa Yi Xue Za Zhi. 21:165–168. 2005.(In Chinese).
18	Pollard H, Khrestchatisky M, Moreau J, Ben-Ari Y and Represa A: Correlation between reactive sprouting and microtubule protein expression in epileptic hippocampus. Neuroscience. 61:773–787. 1994. View Article : Google Scholar : PubMed/NCBI
19	Blurton-Jones M and Laferla FM: Pathways by which Abeta facilitates tau pathology. Curr Alzheimer Res. 3:437–448. 2006. View Article : Google Scholar : PubMed/NCBI
20	Pavia J, de Ceballos ML and Sanchez de la Cuesta F: Alzheimer’s disease: relationship between muscarinic cholinergic receptors, beta-amyloid and tau proteins. Fundam Clin Pharmacol. 12:473–481. 1998.
21	Wang ZF, Li HL, Li XC, et al: Effects of endogenous beta-amyloid overproduction on tau phosphorylation in cell culture. J Neurochem. 98:1167–1175. 2006. View Article : Google Scholar : PubMed/NCBI
22	Ghribi O, Larsen B, Schrag M and Herman MM: High cholesterol content in neurons increases BACE, beta-amyloid, and phosphorylated tau levels in rabbit hippocampus. Exp Neurol. 200:460–467. 2006. View Article : Google Scholar : PubMed/NCBI
23	Peel AL, Sorscher N, Kim JY, Galvan V, Chen S and Bredesen DE: Tau phosphorylation in Alzheimer’s disease: potential involvement of an APP-MAP kinase complex. Neuromolecular Med. 5:205–218. 2004.
24	Kalaria RN, Bhatti SU, Lust WD and Perry G: The amyloid precursor protein in ischemic brain injury and chronic hypoperfusion. Ann NY Acad Sci. 695:190–193. 1993. View Article : Google Scholar : PubMed/NCBI