Juglans mandshurica Maxim extracts exhibit antitumor activity on HeLa cells in vitro

Xin,Nian; Hasan,Murtaza; Li,Wei; Li,Yan

doi:10.3892/mmr.2014.1979

Juglans mandshurica Maxim extracts exhibit antitumor activity on HeLa cells in vitro

Authors:
- Nian Xin
- Murtaza Hasan
- Wei Li
- Yan Li
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Affiliations: School of Life Science, Beijing Institute of Technology, Beijing 100081, P.R. China, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: February 21, 2014 https://doi.org/10.3892/mmr.2014.1979
Pages: 1313-1318

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Abstract

The present study examined the potential application of Juglans mandshurica Maxim extracts (HT) for cancer therapy by assessing their anti‑proliferative activity, reduction of telomerase activity, induction of apoptosis and cell cycle arrest in S phase in HeLa cells. From the perspective of using HT as a herbal medicine, photomicroscopy and florescent microscopy techniques were utilized to characterize the effect of the extracts on telomerase activity and cell morphology. Flow cytometry was employed to study apoptosis and cell cycle of HeLa cells, and DNA laddering was performed. The results showed that HT inhibited cell proliferation and telomerase activity, induced apoptosis and caused S phase arrest of HeLa cells in vitro. HT inhibited HeLa cell proliferation significantly, and the highest inhibition rate was 83.7%. A trap‑silver staining assay showed that HT was capable of markedly decreasing telomerase activity of HeLa cells and this inhibition was enhanced in a time‑ and dose‑dependent manner. Results of a Hoechst 33258 staining assay showed that HeLa cells treated by HT induced cell death. Through DNA agarose gel electrophoresis, DNA ladders of HeLa cells treated with HT were observed, indicating apoptosis. In conclusion, the present study demonstrated that HT exhibited anti‑tumor effects comprising the inhibition of growth and telomerase activity as well as apoptosis and cell cycle arrest in HeLa cells.

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