microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis

  • Authors:
    • Lili Zhou
    • Haitao Bai
    • Chun Wang
    • Daolin Wei
    • Youwen Qin
    • Xiaowei Xu
  • View Affiliations

  • Published online on: March 6, 2014     https://doi.org/10.3892/mmr.2014.2011
  • Pages: 1909-1916
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

microRNA‑125b (miR‑125b) is overexpressed in several types of cancer and contributes to tumor resistance to chemotherapy. The present study investigated the effect of miR‑125b on the resistance of leukemia cell lines to the chemotherapeutic agent daunorubicin (DNR). miR‑125b expression was found to be upregulated in patients who had failed therapy compared with those who demonstrated event‑free survival. The overexpression of miR‑125b was observed to induce DNR resistance in K562, THP‑1 and Jurkat cells by reducing apoptosis, whereas the suppression of miR‑125b was found to enhance DNR cytotoxicity in REH cells. Furthermore, miR‑125b was observed to mediate DNR resistance in leukemia cell lines through decreasing expression of G protein‑coupled receptor kinase 2 and p53‑upregulated modulator of apoptosis, which were shown to be direct targets of miR‑125b using a dual‑luciferase reporter. The present study provides a novel mechanism for understanding leukemia drug resistance and provides a novel method for calculating patient prognosis.
View Figures
View References

Related Articles

Journal Cover

May-2014
Volume 9 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhou L, Bai H, Wang C, Wei D, Qin Y and Xu X: microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis. Mol Med Rep 9: 1909-1916, 2014
APA
Zhou, L., Bai, H., Wang, C., Wei, D., Qin, Y., & Xu, X. (2014). microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis. Molecular Medicine Reports, 9, 1909-1916. https://doi.org/10.3892/mmr.2014.2011
MLA
Zhou, L., Bai, H., Wang, C., Wei, D., Qin, Y., Xu, X."microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis". Molecular Medicine Reports 9.5 (2014): 1909-1916.
Chicago
Zhou, L., Bai, H., Wang, C., Wei, D., Qin, Y., Xu, X."microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis". Molecular Medicine Reports 9, no. 5 (2014): 1909-1916. https://doi.org/10.3892/mmr.2014.2011