Effect of 20-hydroxyeicosatetraenoic acid on biological behavior of human villous trophoblasts and uterine vascular smooth muscle cells

Lu,Shuaijun; Zhu,Changling; Long,Anxiong; Tan,Longyi; Li,Qian; Zhu,Yuli

doi:10.3892/mmr.2014.2017

Effect of 20-hydroxyeicosatetraenoic acid on biological behavior of human villous trophoblasts and uterine vascular smooth muscle cells

Authors:
- Shuaijun Lu
- Changling Zhu
- Anxiong Long
- Longyi Tan
- Qian Li
- Yuli Zhu
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Affiliations: Department of Clinical Laboratory, The Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315000, P.R. China, Department of Gynaecology and Obstetrics, The Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315000, P.R. China, Department of Clinical Laboratory, Baoshan Branch of Shanghai First People's Hospital, Shanghai 200940, P.R. China
Published online on: March 6, 2014 https://doi.org/10.3892/mmr.2014.2017
Pages: 1889-1894

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Abstract

During pregnancy, disorders in uterine spiral artery remodeling (USAR) cause preeclampsia and other diseases. The aim of this study was to investigate the effect of 20-hydroxyeicosatetraenoic acid (20-HETE) on the biological behavior of human villous trophoblasts (HVTs) and human uterine vascular smooth muscle cells (HUVSMCs), and explore the role of 20-HETE in USAR. 20-HETE and its inhibitor HET0016 were used to study migration, invasion and apoptosis in the HVT and HVT-HUVSMC models. 20-HETE inhibited the migration and invasion of HVTs, and inhibited apoptosis in HUVSMCs and HUVSMCs co-cultured with HVTs. 20-HETE had thus obvious effects on the biological behavior of HVTs and HUVSMCs. These effects may cause USAR disorders and vascular dysfunction, leading to preeclampsia.

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1	Shennan AH, Redman C, Cooper C and Milne F: Are most maternal deaths from pre-eclampsia avoidable? Lancet. 379:1686–1687. 2012. View Article : Google Scholar : PubMed/NCBI
2	Young BC, Levine RJ and Karumanchi SA: Pathogenesis of preeclampsia. Annu Rev Pathol. 5:173–192. 2010. View Article : Google Scholar
3	Brosens I, Robertson WB and Dixon HG: The physiological response of the vessels of the placental bed to normal pregnancy. J Pathol Bacteriol. 93:569–579. 1967. View Article : Google Scholar
4	Harris LK: Review: trophoblast-vascular cell interations in early pregnancy: how to remodel a vessel. Placenta. 31:S93–S98. 2010. View Article : Google Scholar : PubMed/NCBI
5	Pijnenborg R, Vercruysse L and Hanssens M: The uterine spiral arteries in human pregnancy: facts and controversies. Placenta. 27:939–958. 2006. View Article : Google Scholar : PubMed/NCBI
6	Whitley GS and Cartwright JE: Cellular and molecular regulation of spiral artery remodelling: lessons from the cardiovascular field. Placenta. 31:465–474. 2010. View Article : Google Scholar : PubMed/NCBI
7	Bulmer JN, Innes BA, Levey J, et al: The role of vascular smooth muscle cell apoptosis and migration during uterine spiral artery remodeling in normal human pregnancy. FASEB J. 26:2975–2985. 2012. View Article : Google Scholar : PubMed/NCBI
8	Guo AM, Scicli G, Sheng J, Falck JC, Edwards PA and Scicli AG: 20-HETE can act as a nonhypoxic regulator of HIF-1alpha in human microvascular endothelial cells. Am J Physiol Heart Circ Physiol. 297:H602–H613. 2009. View Article : Google Scholar : PubMed/NCBI
9	Chen L, Ackerman R and Guo AM: 20-HETE in neovascularization. Prostaglandins Other Lipid Mediat. 98:63–68. 2012. View Article : Google Scholar : PubMed/NCBI
10	Dhanasekaran A, Bodiga S, Gruenloh S, et al: 20-HETE increases survival and decreases apoptosis in pulmonary arteries and pulmonary artery endothelial cells. Am J Physiol Heart Circ Physiol. 296:H777–H786. 2009. View Article : Google Scholar : PubMed/NCBI
11	Pat Kunert M and Drenjancević I: 20-hydroxyeicosatetraenoic acid, endothelial dysfunction and hypertension. Med Glas (Zenica). 8:170–180. 2011.PubMed/NCBI
12	Llinás MT, Alexander BT, Capparelli MF, Carroll MA and Granger JP: Cytochrome P-450 inhibition attenuates hypertension induced by reductions in uterine perfusion pressure in pregnant rats. Hypertension. 43:623–628. 2004.PubMed/NCBI
13	Stec DE, Gannon KP, Beaird JS and Drummond HA: 20-Hydroxyeicosatetraenoic acid (20-HETE) stimulates migration of vascular smooth muscle cells. Cell Physiol Biochem. 19:121–128. 2007. View Article : Google Scholar : PubMed/NCBI
14	Hazan AD, Smith SD, Jones RL, Whittle W, Lye SJ and Dunk CE: Vascular-leukocyte interactions: mechanisms of human decidual spiral artery remodeling in vitro. Am J Pathol. 177:1017–1030. 2010. View Article : Google Scholar : PubMed/NCBI
15	McGiff JC and Quilley J: 20-HETE and the kidney: resolution of old problems and new beginnings. Am J Physiol. 277:R607–R623. 1999.PubMed/NCBI
16	Alexanian A and Sorokin A: Targeting 20-HETE producing enzymes in cancer - rationale, pharmacology, and clinical potential. Onco Targets Ther. 6:243–255. 2013.PubMed/NCBI
17	Guengerich FP and Rendic S: Update information on drug metabolism systems-2009, part I. Curr Drug Metab. 11:1–3. 2010. View Article : Google Scholar : PubMed/NCBI
18	Miyata N, Taniguchi K, Seki T, et al: HET0016, a potent and selective inhibitor of 20-HETE synthesizing enzyme. Br J Pharmacol. 133:325–329. 2001. View Article : Google Scholar : PubMed/NCBI
19	Wang Z, Tang X, Li Y, et al: 20-Hydroxyeicosatetraenoic acid inhibits the apoptotic responses in pulmonary artery smooth muscle cells. Eur J Pharmacol. 588:9–17. 2008. View Article : Google Scholar : PubMed/NCBI
20	Bodiga S, Gruenloh SK, Gao Y, et al: 20-HETE-induced nitric oxide production in pulmonary artery endothelial cells is mediated by NADPH oxidase, H₂O₂, and PI3-kinase/Akt. Am J Physiol Lung Cell and Mol Physiol. 298:L564–L574. 2010. View Article : Google Scholar : PubMed/NCBI
21	Soares MJ, Chakraborty D, Renaud SJ, et al: Regulatory pathways controlling the endovascular invasive trophoblast cell lineage. J Reprod Dev. 58:283–287. 2012. View Article : Google Scholar : PubMed/NCBI
22	Lambert E, Dassé E, Haye B and Petitfrère E: TIMPs as multifacial proteins. Crit Rev Oncol Hematol. 49:187–198. 2004. View Article : Google Scholar
23	Palei AC, Granger JP and Tanus-Santos JE: Matrix metalloproteinases as drug targets in preeclampsia. Curr Drug Targets. 14:325–334. 2013.PubMed/NCBI
24	Lavee M, Goldman S, Daniel-Spiegel E and Shalev E: Matrix metalloproteinase-2 is elevated in midtrimester amniotic fluid prior to the development of preeclampsia. Reprod Biol Endocrinol. 7:852009. View Article : Google Scholar : PubMed/NCBI
25	Ashton SV, Whitley GS, Dash PR, et al: Uterine spiral artery remodeling involves endothelial apoptosis induced by extravillous trophoblasts through Fas/FasL interactions. Arterioscler Thromb Vasc Biol. 25:102–108. 2005.
26	Harris LK, Keogh RJ, Wareing M, et al: Invasive trophoblasts stimulate vascular smooth muscle cell apoptosis by a fas ligand-dependent mechanism. Am J Pathol. 169:1863–1874. 2006. View Article : Google Scholar : PubMed/NCBI
27	Stoneman VE and Bennett MR: Role of Fas/Fas-L in vascular cell apoptosis. J Cardiovasc Pharmacol. 53:100–108. 2009. View Article : Google Scholar : PubMed/NCBI
28	Peppiatt C and Attwell D: Neurobiology: feeding the brain. Nature. 431:137–138. 2004. View Article : Google Scholar : PubMed/NCBI
29	Hoff U, Lukitsch I, Chaykovska L, et al: Inhibition of 20-HETE synthesis and action protects the kidney from ischemia/reperfusion injury. Kidney Int. 79:57–65. 2011. View Article : Google Scholar : PubMed/NCBI
30	Wu CC and Schwartzman ML: The role of 20-HETE in androgen-mediated hypertension. Prostaglandins Other Lipid Mediat. 96:45–53. 2011. View Article : Google Scholar : PubMed/NCBI
31	Jiang H, McGiff JC, Fava C, et al: Maternal and fetal epoxyeicosatrienoic acids in normotensive and preeclamptic pregnancies. Am J Hypertens. 26:271–278. 2013. View Article : Google Scholar : PubMed/NCBI
32	Attwell D, Buchan AM, Charpak S, Lauritzen M, Macvicar BA and Newman EA: Glial and neuronal control of brain blood flow. Nature. 468:232–243. 2010. View Article : Google Scholar : PubMed/NCBI