Caveolin 1 knockdown inhibits the proliferation, migration and invasion of human breast cancer BT474 cells

Wang,Rui; Li,Zhi; Guo,Hui; Shi,Wei; Xin,Yue; Chang,Weilong; Huang,Tao

doi:10.3892/mmr.2014.2018

Caveolin 1 knockdown inhibits the proliferation, migration and invasion of human breast cancer BT474 cells

Authors:
- Rui Wang
- Zhi Li
- Hui Guo
- Wei Shi
- Yue Xin
- Weilong Chang
- Tao Huang
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Affiliations: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
Published online on: March 6, 2014 https://doi.org/10.3892/mmr.2014.2018
Pages: 1723-1728

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Abstract

Previous studies have demonstrated that caveolin 1 acts as a tumor suppressor in breast cancer, however, few studies have demonstrated that caveolin 1 also serves as a tumor promoter in breast cancer. In the present study, caveolin 1 small interfering RNA was used to knock down caveolin 1 expression in order to investigate the association between caveolin 1 and the proliferation and metastatic abilities of human breast cancer BT474 cells. The results revealed that cell proliferation, migration and invasion were attenuated by caveolin 1 knockdown in BT474 cells. Furthermore, caveolin 1 knockdown in BT474 cells arrested cells in the G0/G1 phase and decreased the number of cells in the S phase. In addition, caveolin 1 knockdown decreased the activation of the extracellular signal‑regulated kinase 1/2 pathway and inhibited the expression of cell cycle‑associated proteins (cyclin D1, c‑Fos and β‑catenin), whilst the expression of E‑cadherin was increased. Furthermore, the protein expression of matrix metalloproteinase‑2, ‑9 and ‑1 was also inhibited by caveolin 1 knockdown. In combination, these results demonstrated that caveolin 1 knockdown had a tumor suppressing effect on BT474 cells.

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