Open Access

Anti-inflammatory and anti-proliferative effect of herbal medicines (APR) in RAW264.7 cells

  • Authors:
    • Han-Seok Choi
    • Hye Sook Seo
    • Soon Re Kim
    • Youn Kyung Choi
    • Yong-Cheol Shin
    • Seong-Gyu Ko
  • View Affiliations

  • Published online on: March 10, 2014     https://doi.org/10.3892/mmr.2014.2033
  • Pages: 1569-1574
  • Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The objective of the present study was to analyze the effect of a mixture of medicinal plants [Angelica gigas Nakai, Panax ginseng and Rhus verniciflua Stokes (APR)] on lipopolysaccharide (LPS)-induced inflammatory responses in the murine macrophage cell line RAW264.7. Cells were treated with APR and LPS at various concentrations and indicated times. WST assay, trypan blue assay and quantification of activated cells demonstrated that APR suppressed cell proliferation in a dose-dependent manner. APR induced G1 cell cycle arrest and inhibited the LPS-induced phosphorylation of protein kinase B (AKT), extracellular signal-regulated kinase (ERK), mitogen-activated protein kinase (p38) and necrosis factor κB (NF-κB). APR also suppressed nitric oxide synthase isoform (iNOS) and prostaglandin endoperoxide synthase 2 (Cox-2) messenger ribonucleic acid (mRNA) expression induced by LPS. Furthermore, APR decreased LPS-induced intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential, as well as induced PARP and caspase-3 cleavage, suggesting that APR causes apoptosis. In conclusion, the present study indicated that APR may be advantageous in treating inflammatory disease.
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May-2014
Volume 9 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Choi H, Seo HS, Kim SR, Choi YK, Shin Y and Ko S: Anti-inflammatory and anti-proliferative effect of herbal medicines (APR) in RAW264.7 cells. Mol Med Rep 9: 1569-1574, 2014.
APA
Choi, H., Seo, H.S., Kim, S.R., Choi, Y.K., Shin, Y., & Ko, S. (2014). Anti-inflammatory and anti-proliferative effect of herbal medicines (APR) in RAW264.7 cells. Molecular Medicine Reports, 9, 1569-1574. https://doi.org/10.3892/mmr.2014.2033
MLA
Choi, H., Seo, H. S., Kim, S. R., Choi, Y. K., Shin, Y., Ko, S."Anti-inflammatory and anti-proliferative effect of herbal medicines (APR) in RAW264.7 cells". Molecular Medicine Reports 9.5 (2014): 1569-1574.
Chicago
Choi, H., Seo, H. S., Kim, S. R., Choi, Y. K., Shin, Y., Ko, S."Anti-inflammatory and anti-proliferative effect of herbal medicines (APR) in RAW264.7 cells". Molecular Medicine Reports 9, no. 5 (2014): 1569-1574. https://doi.org/10.3892/mmr.2014.2033