Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia
- Authors:
- Published online on: March 11, 2014 https://doi.org/10.3892/mmr.2014.2038
- Pages: 1715-1722
Abstract
Introduction
General anesthetics act on the central nervous system, mainly the brain and the spinal cord, to induce general anesthesia. General anesthesia has commonly been used in the surgical arena for ~150 years, and its efficacy and safety have been assured by clinical outcomes (1–3). However, little is known regarding its comprehensive influence at the genomic and molecular levels, which is not reflected by mortality and morbidity. Sevoflurane is an inhalational anesthetic frequently used in Japan (4). It has low solubility in blood and tissues, thus there is usually a rapid recovery from anesthesia. Propofol is the most commonly used intravenous anesthetic and is administered as an alkylphenol formulated in a lipid emulsion (5,6). Propofol provides rapid and smooth induction of anesthesia and exhibits rapid clearance from the body.
Due to rapid progress in the field of genomics, microarray technology allows the analysis of genome-wide changes in gene expression in cells and tissues. Several studies regarding general anesthetics, including volatile and intravenous anesthetics, have been performed and they have found that general anesthetics affect gene expression in the rat central nervous system. To elucidate cellular function, exhaustive analyses of the expression of genes (genomics), proteins (proteomics) and metabolites (metabolomics) have been performed in the brain of rats, which is the main target of general anesthetics. In a previous genomic study, the inhalation anesthetic sevoflurane affected the expression of 1.5% of genes in various rat organs, as detected using a microarray analysis (7). General anesthesia altered the expression levels of genes involved in circadian rhythms; persistent suppression of several circadian genes was identified following treatment of rats with the inhalation anesthetic sevoflurane (8) and the intravenous anesthetics propofol and dexmedetomidine (9). In a proteomics study, the protein expression in the rat brain was regulated differentially according to the anesthetic agent used: The inhalation anesthetic sevoflurane or the intravenous anesthetic propofol (10). In a metabolomics study, the inhalation anesthetic isoflurane and the intravenous anesthetic propofol exerted differential effects on the brain metabolism of rats (11). Based on these previous studies, it was hypothesized that gene expression, protein expression and metabolism are different in the rat brain under inhalation anesthesia and intravenous anesthesia.
MicroRNAs (miRNAs) are a class of highly conserved, short, single-stranded, non-coding RNA molecules. miRNAs are 20–24 nucleotides in length and are encoded by the genome. miRNAs bind to imperfectly complementary sequences in the 3′-untranslated region (3′-UTR) of target messenger RNAs (mRNAs) and regulate gene expression at the post-transcriptional level; that is, inhibiting mRNA translation and/or inducing mRNA degeneration (12). A study has shown that miRNAs negatively regulate >30% of mammalian gene expression (13). Furthermore, single miRNAs may bind with hundreds of unique mRNAs, thus altering the expression of multiple cellular pathways (14). miRNAs are important in the central nervous system as they affect various post-transcriptional regulation mechanisms, including cytoskeletal/neuronal growth, cellular metabolism, biosynthesis, stress/death responses, signal transduction and synaptic plasticity (15–18). There is evidence that miRNAs have specific temporal and spatial expression patterns in the mammalian brain (19). However, the gene regulatory networks of the majority of miRNAs in the brain have not yet been fully elucidated.
General anesthetics were developed empirically in clinical settings. This type of anesthetic is essential in producing analgesia, immobility, unconsciousness and memory blockade (amnesia). As certain patients experience unexpected recall of events during surgery, intraoperative amnesia has been a desired endpoint of general anesthesia from the perspectives of the patient and the anesthesiologist. Understanding the mechanisms underlying amnesia is a matter of particular importance. The hippocampus is considered to be an important organ in cognitive function and episodic memory processes and, as reported by Pan et al (20), administration of the inhalation anesthetic sevoflurane resulted in altered gene expression levels of mRNAs that affect memory function in the rat hippocampus.
To the best of our knowledge, no published studies have addressed the association between sevoflurane anesthesia and the expression of miRNAs in the rat hippocampus. It was hypothesized that the miRNAs that influence mRNA expression also alter gene expression levels in the hippocampus under sevoflurane anesthesia. In addition, the inhalation anesthetic sevoflurane and the intravenous anesthetic propofol were predicted to induce different effects on the expression levels of miRNAs in the rat brain. Thus, the aim of the present study was to compare the miRNA expression profiles in the rat hippocampus in response to anesthesia with representative volatile (sevoflurane) and intravenous (propofol) anesthetics. To the best of our knowledge, this is the first attempt to perform an exhaustive profiling analysis of miRNA expression in the hippocampus under general anesthesia.
Materials and methods
Experimental protocol
A previous study model (21–23) on miRNA data acquisition, processing and statistical analysis was used. A flow chart of the experimental protocol is shown in Fig. 1.
Animals
Animal protocols, including the method for euthanasia, were approved by the Animal Experimental Ethics Review Committee of Nippon Medical School (Tokyo, Japan; approval no.: 24-005). All procedures followed the guidelines of the International Association for the Study of Pain (24). Specific pathogen-free male Wistar rats aged six weeks old, (Saitama Experimental Animal Supply Co., Saitama, Japan) were purchased and acclimated under temperature-controlled conditions on a 12-h light/dark cycle, starting at 06:00 and 18:00, respectively, for a week prior to the commencement of experiments. All rats received food and water ad libitum, and the average starting weight of the rats was 250±10 g.
Procedures for administration of anesthetic and termination
During the experiment, each rat was housed in a plastic box (45×32×23 cm), which was large enough for the rat to move and breathe fresh air. The rats were minimally handled in order to reduce stress. The tails of the rats were placed through a small hole in the wall of the box, a catheter was inserted into the tail vein without anesthesia and normal saline was infused continuously at a rate of 1.0 ml/h. The rats were allowed to breathe spontaneously and were supplied with an air-oxygen mixture [fraction of inspired oxygen (FiO2) = 0.40]. Body temperature was maintained at 37.0°C with a heat lamp.
The rats were randomized into three groups (n=7 in each group) and each group received either sevoflurane, propofol or no anesthetic (control group). In the anesthesia groups, the rats were anesthetized with sevoflurane or propofol for 6 h (between 9:00 and 15:00). Rats undergoing inhalational anesthesia (sevoflurane) were placed in plastic boxes supplied with sevoflurane (Sevofrane®; Maruishi Pharmaceutical Co., Ltd., Osaka, Japan; 2.4% gas-air mixture) (25) and 40% oxygen (6 l/min). Normal saline was administered via a venous catheter at a rate of 1.0 ml/h. Rats undergoing intravenous anesthesia (propofol) were housed in plastic boxes supplied with 40% oxygen at a rate of 6 l/min and were administered 1% propofol (Diprivan®; AstraZeneca, Osaka, Japan; 600 μg/kg/min) (26) via a venous catheter (rate of 9.0 ml/h for rats weighing 250 g). In the control group, the rats were not administered anesthetics; however, they were similarly housed in plastic boxes supplied with 40% oxygen at a rate of 6 l/min and normal saline was administered via a venous catheter at a rate of 1.0 ml/h. Rats in the control group were not allowed access to food and water. All rats were allowed to breathe spontaneously. Animals were sacrificed immediately following completion of anesthesia at 15:00. Within 3 mins after decapitation, the whole brain was rapidly removed and the bilateral hippocampus was directly removed from the brain, as described previously (27,28).
Physiological parameters
Physiological variables were measured in three separate groups of rats not used for hippocampal miRNA evaluation (n=7 each), which were treated with sevoflurane, propofol or no anesthetic (control group). For all rats, a catheter was inserted into the tail vein without anesthesia and normal saline was infused continuously at a rate of 1.0 ml/h. During the experiment, each rat was placed in a plastic cage (45×32×23 cm) supplied with an air-oxygen mixture [(FiO2) = 0.40] and allowed to breathe spontaneously. The body temperatures of the rats were maintained at 37.0°C with a heat lamp and their rectal temperatures were measured. As a surgical procedure, the left femoral artery was cannulated to obtain blood samples for measuring the arterial PO2, arterial PCO2, arterial blood pH, heart rate and arterial blood pressure (Table I). Anesthetics were administered as described for the miRNA analysis groups. In the sevoflurane and propofol physiological groups, anesthesia was maintained for an additional 3 or 6 h after surgery. In the control group, the rats were anesthetized by intraperitoneal injection of sodium pentobarbital (50 mg/kg) for the surgery and it was maintained for 3 h. The rats were placed in a rat tunnel in the plastic cages and allowed to recover from the anesthesia. Blood samples were obtained from all five groups.
RNA extraction and miRNA screening test; Taqman Low-Density Arrays (TLDA)
Hippocampal samples were washed twice with cold phosphate-buffered saline, immediately placed in RNAlater solution (Applied Biosystems, Inc., Foster City, CA, USA) and stored at −80°C for one day. Following storage, the samples were defrosted and the RNAlater solution was rapidly separated from the samples by centrifugation (10,000 × g, 4°C, 5 min). Total RNA was extracted from each sample using a mirVana™ miRNA Isolation kit (Applied Biosystems, Inc.) according to the manufacturer’s instructions. RNA quantity and quality were assessed by measuring absorbance using a NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA). Any RNA samples with a 260/280 nm absorbance ratio >1.8 were subjected to quantitative analysis. Total RNA samples containing miRNA were used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
The miRNA expression profiles in the hippocampal samples were analyzed using TLDA Rodent miRNA cards v.3.0 A and B (Applied Biosystems, Inc.), a set of miRNA low-density arrays which enables simultaneous qRT-PCR for 373 preloaded rat miRNA targets and three endogenous controls (Mamm U6, U87 and Y1). All miRNA targets are cataloged in the miRBase database (http://www.mirbase.org/). All procedures were based on those of a previous study (29). Briefly, TLDA was performed as a two-step process. During the first step, 1,200 ng of total RNA per sample was reverse-transcribed using Megaplex RT Primer Pools A and B (Applied Biosystems, Inc.), up to 381 stem-looped primers per pool and a TaqMan miRNA Reverse Transcription kit (Applied Biosystems, Inc.). In the second step, each of the resulting complementary DNA pools was diluted, mixed with TaqMan Universal PCR Master mix (Applied Biosystems, Inc.) and deionized distilled water (Wako Pure Chemical Industries, Ltd., Tokyo, Japan), and loaded into one of the eight fill ports on the TLDA microfluidic card. The cards were briefly centrifuged for 1 min at 315 × g to distribute samples to the multiple wells connected to the fill ports and were then sealed to prevent well-to-well contamination. The cards were processed and analyzed using a 7900HT Fast Real-Time PCR system (Applied Biosystems, Inc.).
Data analysis was performed using DataAssist software v2.0 (Applied Biosystems, Inc.). The resulting data were expressed as threshold cycle (Ct) values, where Ct represents a unitless value defined as the fractional cycle number at which the sample fluorescence signal passes a fixed threshold above baseline. The relative quantities of all the miRNAs were calculated by the comparative Ct method. ΔCt is the difference in Ct values derived from the experimental samples and the controls, and ΔΔCt represents the difference between paired samples, as calculated by the following formula: ΔΔCt = ΔCt of sample following anesthesia − ΔCt of the control group. The expression ratio shows the relative quantity of the target gene (Xtarget) to the control gene (Xcontrol). The fold change was computed by the following formula: Xtarget/Xcontrol = 2−ΔΔCt. The 2−ΔΔCt method is a convenient method for analyzing the relative changes in gene expression from qRT-PCR experiments. Relative quantification (RQ) values (relative levels of miRNA expression) were calculated by this comparative Ct method using RQ Manager version 1.2.1 (Applied Biosystems, Inc.) (30). Graphic displays were visualized as heat map results of hierarchical clustering. Distances between samples and assays were calculated for hierarchical clustering as determined by ΔCt values, using Euclidean distance.
Statistical analysis
Values are expressed as the mean ± standard deviation. Analysis of variance (ANOVA) followed by Tukey-Kramer test was used to compare the physiological data and miRNA expression levels in the sevoflurane anesthesia, propofol anesthesia and control groups. P<0.05 was considered to indicate a statistically significant difference in all tests. Tukey-Kramer test was applied to identify genes and miRNAs that were significantly differentially expressed on exposure to anesthetic agents. ANOVA followed by Tukey-Kramer test was performed using Kyplot software, version 5.0 (KyensLab Incorporated, Tokyo, Japan).
Results
Anesthesia of rats
Following induction of sevoflurane or propofol anesthesia, all rats lost consciousness within 5 min, with loss of corneal reflexes and the withdrawal response to pain. No rats moved spontaneously or died until the end of anesthesia. Data for all animals were used.
Physiological data
The possible effects of anesthesia on a number of physiological parameters were investigated in three separate groups of rats. Fig. 1 shows a flow chart of the experimental protocol. The physiological data for the treatment groups during anesthesia were compared with those of the control group, in which no anesthesia was administered following the termination of anesthesia for the cannulation of the femoral artery. Low mean blood pressure and high PCO2 were observed following the administration of anesthetic drugs. However, no significant differences in the physiological parameters among the administration groups and control group were identified (Table I). Hypoxia, hyper/hypocapnia, hypotension or hypothermia were not observed in any of the three groups.
miRNA screening test: TLDA
In order to identify suitable endogenous controls, three different candidate miRNAs were analyzed for variance in gene expression with DataAssist software v2.0. The statistical method ranked the candidate endogenous control genes with an excellent correlation of raw stability values. The most stably expressed Y1 was selected as the endogenous control and the relative miRNA expression levels were normalized against Y1. Ct values >35 indicated that their expression levels were too low for accurate analysis. The miRNA expression Ct value was therefore standardized at ≤35.
The results of the TLDA analysis of the hippocampi are shown in Tables II–IV (n=7 for each group; significance level, P<0.05). There were 750 miRNAs on the TLDA plate; 373 miRNAs were associated with rats and the rest with mice. TLDA analysis revealed 279 expressed miRNAs (74.8%), which were expressed in the sevoflurane, propofol and control groups. Significant differences were observed in the levels of 33 of the 279 expressed miRNAs (11.8%) among the three groups in response to the anesthetic agents, and when compared with the control group, significant differences were found in 26 of the 279 expressed miRNAs (9.3%). Following sevoflurane anesthesia, the levels of four miRNAs were significantly increased and those of 12 were significantly reduced compared with those of the control group. By contrast, following propofol anesthesia, the levels of 11 miRNAs were significantly reduced but no miRNAs exhibited significantly elevated levels. Of the total 33 significantly differentially expressed miRNAs, one miRNA was common to both anesthetics, whereas 14 miRNAs were significantly differentially expressed (Tables II–IV).
![]() | Table IIDifferential expression of miRNAs between the sevoflurane anesthesia and control groups using TLDA. |
![]() | Table IVDifferential expression of miRNAs between the sevoflurane and propofol anesthesia groups using TLDA. |
Cluster analysis from TLDA data is shown in Fig. 2. Significantly differentially expressed miRNAs were converted into a heat map in order to improve the understanding of the miRNA expression patterns in each group. Heat maps for the calculated clusters are commonly used gene-expression analyses and are constructed for visualization of gene expression levels. In the present study, heat maps illustrated the gene expression levels from the hippocampal samples following treatment with anesthetics using ΔCT values. The color intensity on the heat map corresponds to the absolute intensity of the miRNA expression. Each column represents a sample and each row shows miRNA in the heat map. By individually clustering columns and rows, the heat map simultaneously presents the separate samples and miRNA clustering in one graphic. The dendrograms of clustering analysis for the samples and miRNAs are shown to the top and left of the heat map. This indicated the relatedness of the samples as determined by the overall miRNA expression values. The dendrogram separates into three main branches: The sevoflurane anesthesia, the propofol anesthesia and the control groups.
Discussion
The present study demonstrated that sevoflurane and propofol anesthesia induced numerous changes in the miRNA expression levels of the rat hippocampus. Using TLDA data, the hierarchical clustering with heat map presented the semantic similarities and expression patterns of miRNA. Hierarchical clustering separated the samples into three distinct branches in the dendrogram: The sevoflurane anesthesia group, the propofol anesthesia group and the control group (no anesthetic). These findings indicate that the miRNA expression patterns with general anesthetic treatment were distinct from those of the control group. In addition, the inhalation anesthetic sevoflurane and the intravenous anesthetic propofol exerted different effects on the miRNA expression in the rat hippocampus.
In the present study, experiments were conducted using the inhaled anesthetic sevoflurane and the intravenous anesthetic propofol. Sevoflurane was selected as it is one of the most commonly used inhaled clinical anesthetic agents in Japan (4). Propofol was selected as it is a widely used intravenous anesthetic in everyday clinical practice in a number of countries. The two drugs have been used extensively in previous studies concerning the effects of anesthetic agents on gene expression (7–9), protein expression (10) and metabolites (11) in the rat brain. Changes in the miRNA expression levels in the hippocampi of rats anesthetized with one minimum alveolar concentration of inhaled anesthetics (sevoflurane at 2.4%; 22) or with continuous infusion of intravenous anesthetics (propofol at 600 μg/kg/min; 23) were investigated in the present study. Infusion of propofol at 600 μg/kg/min is the median effective dose for rats (26). The two doses were selected since lower doses may not induce adequate anesthesia and higher doses may produce hemodynamic changes (10,22) that may independently alter hippocampal miRNA expression levels.
A number of miRNAs determined to be differentially expressed in the present study are known to be involved in stem cell self-renewal, synaptic plasticity and memory consolidation in the rat hippocampus. For example, miR-125b, a neuronal miRNA, is important in proliferation and differentiation of neural stem cells (31). miRNA-181a is rich in mature nerve cells and bioinformatic analysis has revealed that the cAMP response element-binding protein 1 (CREB1) mRNA 3′-UTR contains a complementary sequence to the miR-181a seed region. CREB1 is a key nuclear factor highly expressed in hippocampal neurons on which numerous signaling pathways converge (32–34). In the present study, the expression levels of these miRNAs were found to be significantly affected by sevoflurane and/or propofol anesthesia; therefore, general anesthesia may affect these cell signaling pathways, including synaptic plasticity and memory consolidation. In addition, the changes in the levels of miRNA may be attenuated following general anesthesia.
It was hypothesized that sevoflurane and propofol anesthesia induce different expression patterns of miRNA in the rat hippocampus. Sevoflurane and propofol induce general anesthesia but have different mechanisms of anesthetic action. Single miRNAs may bind with hundreds of unique mRNAs, thus regulating the expression of multiple cellular pathways, and individual miRNAs reciprocally act on mRNA. The TLDA data of the present study were not satisfactory to determine the validity of this hypothesis since they provide only a snapshot of the changes in miRNA expression patterns. Pan et al (20) showed that sevoflurane anesthesia induces changes in the mRNA expression levels in the rat hippocampus, which may be associated with memory impairment or other neural disorders. Although changes in the levels of mRNA and miRNA expression are likely to affect subsequent protein expression, there is a lack of correlation between them. The final functional entities in cells are not mRNAs or miRNAs, but proteins that undergo numerous post-translational modifications, thereby controlling cellular mechanisms (35). Investigation of the action mechanisms of general anesthetics is a noteworthy field and further studies using molecular approaches are required for the elucidation of these mechanisms.
Due to advances in high-throughput technologies and bioinformatics, there are several methods for gene expression profiling. The results of microarray analyses are commonly validated using qRT-PCR, which is the most sensitive and reproducible method for quantifying gene expression. Single qRT-PCR assays and TLDA analyses rely on identical amplification technologies. Single qRT-PCR is a reliable method for the analysis of miRNA expression as the reactions are run in triplicate; by contrast, TLDA analyses are run individually. Hui et al (36) demonstrated that the technical reproducibility of TLDAs was high (intrasample correlations >0.9, accuracy 92.8%). Furthermore, Mees et al (37) demonstrated that TLDA and single qRT-PCR correlated well with regards to the quantity and quality of the measured miRNAs. The TLDA analysis of the present study has the same procedures as those of previous studies (21,22,38). In these studies, the single qRT-PCR results of individual miRNA were equivalent to the TLDA analysis results. These results confirmed that the TLDA analysis data of the present study were as accurate as those of single qRT-PCR and that the TLDA data were reliable.
The present study has certain limitations. The study was conducted in rats and innate differences in gene expression in different species of animals mean that extrapolation of results from rats to other species is difficult. Additionally, miRNA microarray analyses were conducted using whole hippocampal samples. The hippocampus is composed of the dentate gyrus and the cornu ammonis. The cornu ammonis is separated into three predominant divisions: CA1, CA2 and CA3, but the results of the present study only reflected the whole hippocampal miRNA expression pattern. For further analyses, localization of gene-expression changes is required. Furthermore, a large number of miRNAs were measured; therefore, it is possible that certain results were false positives.
In conclusion, the data from the present study indicated changes in the miRNA expression levels in the rat hippocampus under general anesthesia. When compared with that of the control group, the inhalation anesthetic sevoflurane and the intravenous anesthetic propofol exerted differential effects on miRNA expression. In subsequent studies, the time-dependent changes in the levels of miRNA expression under general anesthesia may be further investigated, along with the post-anesthetic influence of miRNA at various time points.
Acknowledgements
This study was supported by a Grant-in-Aid for Science Research (C) (project no. 20591820) from the Japan Society for the Promotion of Science (Tokyo, Japan).
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