MicroRNA‑518d regulates PPARα protein expression in the placentas of females with gestational diabetes mellitus

Zhao,Chun; Zhang,Ting; Shi,Zhonghua; Ding,Hongjuan; Ling,Xiufeng

doi:10.3892/mmr.2014.2058

MicroRNA‑518d regulates PPARα protein expression in the placentas of females with gestational diabetes mellitus

Authors:
- Chun Zhao
- Ting Zhang
- Zhonghua Shi
- Hongjuan Ding
- Xiufeng Ling
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Affiliations: State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China
Published online on: March 17, 2014 https://doi.org/10.3892/mmr.2014.2058
Pages: 2085-2090

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Abstract

The placenta is thought to have a critical role in the pathogenesis of gestational diabetes mellitus (GDM), as GDM‑associated complications resolve following delivery. Placenta‑specific microRNAs (miRNAs) may contribute to the pathology of the development of GDM. The aim of the present study was to evaluate whether the placenta‑specific miR‑518d contributes to the development of GDM. It was revealed that miR‑518d expression was higher in placentas taken from patients with GDM compared with control placentas, whereas the protein levels of the predicted miR‑518d target gene, peroxisome proliferator‑activated receptor‑α (PPARα), were lower in placentas from patients with GDM compared with those from control subjects. It was also demonstrated that PPARα was a direct target of miR‑518d with a specific binding site at the seed sequence, which determines target specificity. In the placentas of females with GDM increased levels of miR‑518d were negatively correlated with the levels of PPARα protein. As PPARα dysregulation may be related to the development of GDM, it is suggested that upregulation of miR‑518d may be associated with the pathogenesis of GDM via an effect on the regulation of PPARα expression.

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