MG132 reverse the malignant characteristics of hypopharyngeal cancer

  • Authors:
    • Juke Ma
    • Liang Yu
    • Jiajun Tian
    • Yakui Mu
    • Zhenghua Lv
    • Jidong Zou
    • Jianfeng Li
    • Haibo Wang
    • Wei Xu
  • View Affiliations

  • Published online on: April 1, 2014     https://doi.org/10.3892/mmr.2014.2103
  • Pages: 2587-2591
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Abstract

In order to reverse the malignant characteristics of hypopharyngeal cancer, the proteasome inhibitor MG132 was introduced into FaDu/T cells and the mechanisms underlying its effects were investigated. The multi-drug resistance (MDR) sensitivities of FaDu/T and FaDu/T-MG132 cancer cells to several chemotherapeutics were investigated by a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay. Apoptosis was measured by staining cells with Annexin V and propidium iodide (PI) double staining. Reverse transcription‑polymerase chain reaction and western blot analysis were conducted to detect mRNA and corresponding protein levels of the MDR- and apoptosis‑related genes P-glycoprotein (P-gp), caspase-3, Bcl-2 and Bax. The nuclear protein of nuclear factor κ-light-chain-enhancer of activated B cells. (NF-κB) and p53 were also investigated via western blot analysis. Compared with FaDu/T cells, the drug resistance of FaDu/T + MG132 cells to cisplatin (DDP), 5-fluorouracil (5-FU), doxorubicin (Dox) and vincristine (VCR) decreased. With increased expression of caspase-3 and Bax and decreased expression of Bcl-2, the anti‑apoptotic ability markedly decreased in FaDu/T + MG132 cells. P-gp and NF-κB significantly decreased; however, p53 increased in FaDu/T + MG132 cells. These results suggested that the proteasome inhibitor MG132 reversed the malignant characteristics of FaDu/T by enhancing apoptosis and inhibiting P-gp. MG132 was also able to inhibit the nuclear translocation of NF-κB and increase the expression of p53.
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June-2014
Volume 9 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Ma J, Yu L, Tian J, Mu Y, Lv Z, Zou J, Li J, Wang H and Xu W: MG132 reverse the malignant characteristics of hypopharyngeal cancer. Mol Med Rep 9: 2587-2591, 2014
APA
Ma, J., Yu, L., Tian, J., Mu, Y., Lv, Z., Zou, J. ... Xu, W. (2014). MG132 reverse the malignant characteristics of hypopharyngeal cancer. Molecular Medicine Reports, 9, 2587-2591. https://doi.org/10.3892/mmr.2014.2103
MLA
Ma, J., Yu, L., Tian, J., Mu, Y., Lv, Z., Zou, J., Li, J., Wang, H., Xu, W."MG132 reverse the malignant characteristics of hypopharyngeal cancer". Molecular Medicine Reports 9.6 (2014): 2587-2591.
Chicago
Ma, J., Yu, L., Tian, J., Mu, Y., Lv, Z., Zou, J., Li, J., Wang, H., Xu, W."MG132 reverse the malignant characteristics of hypopharyngeal cancer". Molecular Medicine Reports 9, no. 6 (2014): 2587-2591. https://doi.org/10.3892/mmr.2014.2103