Application of two-dimensional electrophoresis and mass spectrometry to screen endometriosis‑related proteins

Tuerxun,Hanikezi; Zhang,Yanmei; Ji,Fei; Aili,Aixingzi; Yang,Xinhua; Ding,Yan

doi:10.3892/mmr.2014.2142

Application of two-dimensional electrophoresis and mass spectrometry to screen endometriosis‑related proteins

Authors:
- Hanikezi Tuerxun
- Yanmei Zhang
- Fei Ji
- Aixingzi Aili
- Xinhua Yang
- Yan Ding
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Affiliations: Gynecology Department, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China, Department of Obstetrics, Xinjiang Uygur Autonomous Region People's Hospital, Urumqi, Xinjiang 830001, P.R. China, Department of Gynecology, Shanghai Dongfang Hospital, Shanghai 200120, P.R. China
Published online on: April 15, 2014 https://doi.org/10.3892/mmr.2014.2142
Pages: 95-100

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Abstract

The aim of the present study was to detect the differences in the protein expression between Uyghur females with or without endometriosis (EM). The two dimensional (2D) gel images of proteins extracted from the serum of Uyghur and Han females with EM and the controls were analyzed using Phoretix 2D software, and the differentially expressed proteins were identified primarily by database query. Having compared the reproducible 2D gel images of proteins from the serum of Uihgur and Han females with and without EM, 13 differentially expressed proteins were obtained from Uyghur females and eight differentially expressed proteins were obtained from Han females. The present study demonstrated ethnic differences in gene and protein expression between Uyghur and Han females with EM.

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