Novel curcumin analogue IHCH exhibits potent anti‑proliferative effects by inducing autophagy in A549 lung cancer cells

  • Authors:
    • Guang‑Zhou Zhou
    • Su‑Li Xu
    • Gang‑Chun Sun
    • Xiao‑Bing Chen
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  • Published online on: April 24, 2014     https://doi.org/10.3892/mmr.2014.2183
  • Pages: 441-446
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Abstract

Curcumin is a natural polyphenolic compound that exhibits strong antioxidant and anticancer activities; however, low bioavailability has restricted its application in chemotherapeutic trials. The present study aimed to investigate the anticancer effect of the novel curcumin derivative 2E,6E‑2‑(1H‑indol‑3‑yl) methylene)‑6‑(4‑hydroxy‑3‑methoxy benzylidene)‑cyclohexanone (IHCH) on A549 lung cancer cells. Cells were treated with IHCH at different concentrations (1‑40 µM) for different time periods (1‑36 h). Microscopic analysis revealed that IHCH inhibited A549 cell growth and induced the formation of characteristic autophagolysosomes in a dose‑ and time‑dependent manner. Furthermore, the inhibitory rate of IHCH (40 µM) on A549 cell viability was 77.34% after 36 h of treatment. Acridine orange staining revealed an increase in autophagic vacuoles in the IHCH‑treated A549 cells. Monodansylcadaverine staining was used to analyze autophagy rate. Immunocytochemistry revealed an increase in light chain (LC) 3 protein expression in the IHCH‑treated cells and western blot analysis detected the conversion of LC3‑I to LC3‑II, as well as the recruitment of LC3 to autophagosomes in the cytoplasmatic compartment, suggesting the occurrence of autophagy. These findings show that IHCH induced autophagy in A549 cells, which is a novel cell death mechanism induced by curcumin derivatives.
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July-2014
Volume 10 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhou GZ, Xu SL, Sun GC and Chen XB: Novel curcumin analogue IHCH exhibits potent anti‑proliferative effects by inducing autophagy in A549 lung cancer cells. Mol Med Rep 10: 441-446, 2014.
APA
Zhou, G., Xu, S., Sun, G., & Chen, X. (2014). Novel curcumin analogue IHCH exhibits potent anti‑proliferative effects by inducing autophagy in A549 lung cancer cells. Molecular Medicine Reports, 10, 441-446. https://doi.org/10.3892/mmr.2014.2183
MLA
Zhou, G., Xu, S., Sun, G., Chen, X."Novel curcumin analogue IHCH exhibits potent anti‑proliferative effects by inducing autophagy in A549 lung cancer cells". Molecular Medicine Reports 10.1 (2014): 441-446.
Chicago
Zhou, G., Xu, S., Sun, G., Chen, X."Novel curcumin analogue IHCH exhibits potent anti‑proliferative effects by inducing autophagy in A549 lung cancer cells". Molecular Medicine Reports 10, no. 1 (2014): 441-446. https://doi.org/10.3892/mmr.2014.2183