Mast cell activation and response to tolterodine in the rat urinary bladder in a chronic model of intravesical protamine sulfate and bacterial endotoxin-induced cystitis

Choi,Bo-Hwa; Jin,Long-Hu; Kim,Khae-Hawn; Han,Jee-Young; Kang,Ju‑Hee; Yoon,Sang-Min; Park,Chang-Shin; Lee,Tack

doi:10.3892/mmr.2014.2262

Mast cell activation and response to tolterodine in the rat urinary bladder in a chronic model of intravesical protamine sulfate and bacterial endotoxin-induced cystitis

Authors:
- Bo-Hwa Choi
- Long-Hu Jin
- Khae-Hawn Kim
- Jee-Young Han
- Ju‑Hee Kang
- Sang-Min Yoon
- Chang-Shin Park
- Tack Lee
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Affiliations: Department of Pharmacology, Inha University College of Medicine by BK 21 Project, Incheon 400-711, South Korea, Department of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China, Department of Urology, Gachon University Gil Medical Center, Incheon 405-760, South Korea, Department of Pathology, Inha University College of Medicine by BK 21 Project, Incheon 400-711, South Korea, Department of Urology, Inha University College of Medicine by BK 21 Project, Incheon 400-711, South Korea
Published online on: May 22, 2014 https://doi.org/10.3892/mmr.2014.2262
Pages: 670-676

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Abstract

The aim of the present study was to use an animal model of interstitial cystitis (IC) in order to investigate the histology and function of the bladder, with a particular focus on mast cell degranulation and response to detrusor overactivity (DO) to tolterodine. A total of 18 female Sprague‑Dawley rats were used. In 12 rats, lipopolysaccharide (LPS) was intravesically instilled following the induction of IC by protamine sulfate (PS) and six rats were subjected to sham instillations. Following 1 month, cystometry was performed. The effects of tolterodine were tested in half of the animals with IC. All rats in the IC group demonstrated DO during the filling phase and no significant changes in the frequency or pressure compared with that following tolterodine injection were identified. Histological examination revealed a significant increase in the total number of infiltrated mast cells in IC rats compared with that in the sham rats (P<0.05). Degranulated mast cells were evident in 80% of rats with IC; however, they were not apparent in the sham rats. Urinary bladder inflammation, similar to that in human IC in terms of degranulated mast cells and bladder function, was induced in rats. The animal model used in the present study provided insight into the pathophysiological mechanisms underlying the ineffectiveness of anticholinergics in patients with overlapping IC and overactive bladder (OAB).

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