Measurement of hydroxyproline in collagen with three different methods

Qiu,Bing; Wei,Fengxiang; Sun,Xiuzhi; Wang,Xi; Duan,Binhong; Shi,Chunlin; Zhang,Jianying; Zhang,Jiye; Qiu,Wenliang; Mu,Wenling

doi:10.3892/mmr.2014.2267

Measurement of hydroxyproline in collagen with three different methods

Authors:
- Bing Qiu
- Fengxiang Wei
- Xiuzhi Sun
- Xi Wang
- Binhong Duan
- Chunlin Shi
- Jianying Zhang
- Jiye Zhang
- Wenliang Qiu
- Wenling Mu
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Affiliations: Department of Gastroenterology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China, Genetics Laboratory Shenzhen Longgang District Maternity and Child Healthcare Hospital, Shenzhen, Guangdong 518124, P.R. China, Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Endocrinology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China
Published online on: May 23, 2014 https://doi.org/10.3892/mmr.2014.2267
Pages: 1157-1163

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Abstract

Determination of the 4-hydroxy-l-proline (hydroxyproline) concentration may provide useful information for the diagnosis and prognosis of diseases caused by disorders of collagen metabolism. The objective of the present study was to apply liquid chromatography-mass spectrometry (LC-MS) to assess the hydroxyproline concentration. The hydroxyproline concentration in lung and liver tissues measured by LC-MS was compared with values obtained by a colorimetric method, as well as a fluorescence method using high-performance liquid chromatography (HPLC) from previous studies by our group. The determination of the hydroxyproline concentration by LC-MS was improved as compared with that using the colorimetric and HPLC methods, due to its simplicity, high sensitivity (pg level) and short separation time. These results suggested that utilizing the LC-MS method for measuring the hydroxyproline concentration would be advantageous for the diagnosis of diseases associated with abnormalities of collagen metabolism.

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